Cloned (Comment) | Organism |
---|---|
gene lacC, codon-optimized, recombinant expression in Escherichia coli strain BL21(DE3) | Lactococcus lactis subsp. lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lactococcus lactis subsp. lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-tagatose 6-phosphate | Lactococcus lactis subsp. lactis | - |
ADP + D-tagatose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis subsp. lactis | P23391 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Lactococcus lactis subsp. lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-tagatose 6-phosphate | - |
Lactococcus lactis subsp. lactis | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
additional information | enzyme efficient biocatalytic synthesis system is developed for the phosphorylation of D-tagatose 6-phosphate at its C1-position using the recombinant D-tagatose 6-phosphate kinase and the phosphoenolpyruvate/pyruvate kinase-system for ATP regeneration. This straightforward and scalable one-step biocatalytic synthesis of D-tagatose 1,6-diphosphate is successfully scaled up to the gram scale. Method evaluation and optimization, production analysis by NMR spectroscopy, overview | Lactococcus lactis subsp. lactis | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
D-tagatose 6-phosphate kinase | - |
Lactococcus lactis subsp. lactis |
Lacc | - |
Lactococcus lactis subsp. lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lactococcus lactis subsp. lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Lactococcus lactis subsp. lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Lactococcus lactis subsp. lactis |
General Information | Comment | Organism |
---|---|---|
metabolism | D-tagatose 1,6-diphosphate is an important metabolite which can be formed via the classical tagatose 6-phosphate pathway or via the tagatose 1-phosphate pathway | Lactococcus lactis subsp. lactis |
physiological function | enzyme LacC catalyses the efficient biocatalytic synthesis of D-tagatose 1,6-diphosphate via phosphorylation of D-tagatose 6-phosphate | Lactococcus lactis subsp. lactis |