Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
native enzyme and L124M, L125M double mutant expressed in Escherichia coli BL21(DE3) | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
high resolution structures of D-tagatose-6-phosphate kinase (LacC) in two crystal forms. The structures define LacC in apoform, in binary complexes with ADP or the cofactor analogue adenosine 5'-(beta,gamma-imino)triphosphate, and in a ternary complex with adenosine 5'-(beta,gamma-imino)triphosphate and D-tagatose-6-phosphate. LacC adopts a closed structure required for phosphoryl transfer only when both substrate and co-factor are bound | Staphylococcus aureus |
native and L124M, L125M double mutant crystallized by hanging drop vapor diffusion method in apoform, in binary complexes with ADP or the cofactor analogue AMP-PNP, and in a ternary complex with AMP-PNP and D-tagatose-6-phosphate, wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
L124M/L125M | wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, two ions bound to the active site when substrate and cofactor are bound | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-tagatose 6-phosphate | Staphylococcus aureus | second step of the D-tagatose-6-phosphate pathway which is the direct catabolic pathway for lactose | ADP + D-tagatose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P0A0B9 | - |
- |
Purification (Comment) | Organism |
---|---|
native and mutant protein from Escherichia coli | Staphylococcus aureus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate | mechanism suggested | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-tagatose 6-phosphate | second step of the D-tagatose-6-phosphate pathway which is the direct catabolic pathway for lactose | Staphylococcus aureus | ADP + D-tagatose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration data, crystal structure analysis, two monomers associate via interactions between their lid domains, which come together to form a beta-barrel structure known as a beta-clasp | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
D-tagatose-6-phosphate kinase | - |
Staphylococcus aureus |
Lacc | - |
Staphylococcus aureus |
Lacc | belongs to the pfkB family of carbohydrate kinases, which form a subset of the ribokinase superfamily | Staphylococcus aureus |
phosphotagatokinase | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |