Literature summary for 2.7.1.138 extracted from

Chen, W.Q.; Graf, C.; Zimmel, D.; Rovina, P.; Krapfenbauer, K.; Jaritz, M.; Parker, P.J.; Lubec, G.; Bornancin, F.
Ceramide kinase profiling by mass spectrometry reveals a conserved phosphorylation pattern downstream of the catalytic site (2010), J. Proteome Res., 9, 420-429.

Search for more literature for 2.7.1.138

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in COS-1 cells	Homo sapiens
recombinant full-length hCERK is obtained from baculovirus-infected Sf9 cells as a glutathione-S-transferase fusion	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S340A	by site directed mutagenesis, using of Triton X-100 as lysis buffer results in 15% activity decrease in the mutant protein compared with the wild type enzyme, when octylglucoside is used instead of Triton X-100 for cell lysis, activity is reduced in the S340A mutant protein which reaches only 15% of wild type activity	Homo sapiens
S340D	by site directed mutagenesis, intermediate recovery of 16% is observed for the mutant protein	Homo sapiens
S427A	by site directed mutagenesis, activity in the S427A mutant protein amounts to only 30% of that of wild type enzyme	Homo sapiens
S427D	by site directed mutagenesis, mutant protein displays 50% of wild type activity	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + ceramide	Homo sapiens	-	ADP + ceramide 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8TCT0	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation of serine residues at positions 340 and 408, putative additional phosphorylation site at serine 427 lying in the calcium/calmodulin domain, preventing phosphorylation at S340 results in CERK instability	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
by affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + ceramide	-	Homo sapiens	ADP + ceramide 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
ceramide kinase	-	Homo sapiens
CERK	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)