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Literature summary for 2.7.1.138 extracted from

  • Mitsutake, S.; Igarashi, Y.
    Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium-sensor (2005), J. Biol. Chem., 280, 40436-40441.
    View publication on PubMed
Search for more literature for 2.7.1.138

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin binding site is located at residues 422-435, which form a amphipathic helical wheel, motif 1-8-14, calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium-sensor Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in rat basophilic leukemia RBL-2H3 cells, expression of wild-type and mutant enzymes in CHO cells Mus musculus

Protein Variants

Protein Variants Comment Organism
F429R site-directed mutagenesis, weak binding of calmodulin compared to the wild-type enzyme Mus musculus
F431R site-directed mutagenesis, no binding of calmodulin in contrary to the wild-type enzyme Mus musculus
L422R site-directed mutagenesis, similar binding of calmodulin compared to the wild-type enzyme Mus musculus
L422R/F429R site-directed mutagenesis Mus musculus
L435R site-directed mutagenesis, no binding of calmodulin in contrary to the wild-type enzyme Mus musculus
additional information construction of an enzyme mutant which shows loss of activity but contains the calmodulin binding motif, expression of the mutant inhibits Ca2+-dependent ceramide 1-phosphate formation Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
KN-93
-
 Mus musculus
W-7 calmodulin antagonist Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ completely dependent on, regulatory function for enzyme activity, calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium-sensor Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ceramide Mus musculus
-
 ADP + ceramide 1-phosphate
-
 ?
additional information Mus musculus ceramide kinase is a calcium-sensor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ceramide
-
 Mus musculus ADP + ceramide 1-phosphate
-
 ?
additional information ceramide kinase is a calcium-sensor Mus musculus ?
-
 ?
additional information the enzyme forms intracellular complexes with calmodulin Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
CERK
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus