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Literature summary for 2.7.1.138 extracted from

  • Baumruker, T.; Bornancin, F.; Billich, A.
    The role of sphingosine and ceramide kinases in inflammatory responses (2005), Immunol. Lett., 96, 175-185.
    View publication on PubMed
Search for more literature for 2.7.1.138

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is induced by the calcium triggers interleukin-1beta and IgE/Ag due to calcium mobilization Homo sapiens
Phorbol esters e.g. PMA Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, expression in HeLa and COS-1 cells, expression of GFP-tagged enzyme in HUVEC and COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information siRNA to the gene encoding the enzyme for downregulation blocks arachidonic acid release and subsequent prostaglandin E2 production after stimulation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
C2-ceramide
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 ATP recombinant enzyme Homo sapiens
0.187
-
 ceramide recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
Golgi apparatus
-
 Homo sapiens 5794
-
membrane bound Homo sapiens 16020
-
additional information subcellular localization may be dependent on the cell type Homo sapiens
-
-
synaptic vesicle
-
 Homo sapiens 8021
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Homo sapiens
Mg2+ activation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ceramide Homo sapiens
-
 ADP + ceramide 1-phosphate ceramide 1-phosphate is a lipid signalling molecule involved in regulation of many physiological processes related to cell proliferation, apoptosis, and inflammation, most of them in concert with sphingosine 1-phosphate, ceramide 1-phosphate might act via extracellular cell surface receptors or intracellularly as second messenger ?
additional information Homo sapiens the enzyme does not play a role in neutrophil priming, the enzyme regulates the activity and translocalization of cytosolic phospholipase isozyme A2alpha, overview, the enzyme is involved in prostaglandin biosynthesis, i.e. intracellular arachidonic acid production, which is blocked by enzyme gene silencing ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein the enzyme is myristoylated at the N-terminus within the pleckstrin homology PH domain, required for activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
additional information the enzyme is expressed in all or nearly all tissues and cell types Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + C16-ceramide lower activity Homo sapiens ADP + C16-ceramide 1-phosphate
-
 ?
ATP + C18-ceramide lower activity Homo sapiens ADP + C18-ceramide 1-phosphate
-
 ?
ATP + C8-ceramide best substrate Homo sapiens ADP + C8-ceramide 1-phosphate
-
 ?
ATP + C8-dihydroceramide high activity Homo sapiens ADP + C8-dihydroceramide 1-phosphate
-
 ?
ATP + C8-phytoceramide high activity Homo sapiens ADP + C8-phytoceramide 1-phosphate
-
 ?
ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate ceramide 1-phosphate is a lipid signalling molecule involved in regulation of many physiological processes related to cell proliferation, apoptosis, and inflammation, most of them in concert with sphingosine 1-phosphate, ceramide 1-phosphate might act via extracellular cell surface receptors or intracellularly as second messenger ?
ATP + ceramide the enzyme is specific for the cramide backbone, acylation of amino group in position 2 is required Homo sapiens ADP + ceramide 1-phosphate
-
 ?
additional information the enzyme does not play a role in neutrophil priming, the enzyme regulates the activity and translocalization of cytosolic phospholipase isozyme A2alpha, overview, the enzyme is involved in prostaglandin biosynthesis, i.e. intracellular arachidonic acid production, which is blocked by enzyme gene silencing Homo sapiens ?
-
 ?
additional information no activity with GTP, no activity with sphingosine and sphinganine, and with diacylglycerol Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme possesses an N-terminal pleckstrin homology PH domain, residues 32-121, and a C-terminal region which contains a Ca2+/calmodulin binding domain, residues 378-455, that flanks the catalytic domain, residues 124-433, the enzyme also contains 5 sequences stretches C1-C5 typical for lipid kinases Homo sapiens

Synonyms

Synonyms Comment Organism
CERK
-
 Homo sapiens
More the enzyme belongs to the subfamily of diacylglyceride kinases DAGKs Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 sharp optimum Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP GGDG motif binding site is located in the C2-subdomain Homo sapiens
additional information no activity with GTP Homo sapiens