Cloned (Comment) | Organism |
---|---|
expression of C-terminally FLAG-tagged and of EGFP-tagged wild-type and mutant enzymes in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G2A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic vesicle | - |
Homo sapiens | 31410 | - |
membrane | bound to, probably via the pleckstrin homology domain of the enzyme | Homo sapiens | 16020 | - |
additional information | the enzyme is involved in vesicular trafficking and subcellular translocation involving its pleckstrin homology domain, the enzyme co-localizes with its substrate, overview | Homo sapiens | - |
- |
perinuclear space | - |
Homo sapiens | - |
- |
plasma membrane | - |
Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 3 mM CaCl2 | Homo sapiens | |
NaCl | at 50 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
ATP + ceramide | the pleckstrin homology domain is essential for activity, binding of liposomes | Homo sapiens | ADP + ceramide 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | subdomain structure of the enzyme, possessing a pleckstrin homology domain, a DAGK-like catalytic domain, and a Ca2+/calmodulin binding domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CERK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |