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Literature summary for 2.7.1.138 extracted from

  • Sugiura, M.; Kono, K.; Liu, H.; Shimizugawa, T.; Minekura, H.; Spiegel, S.; Kohama, T.
    Ceramide kinase, a novel lipid kinase. Molecular cloning and functional characterization (2002), J. Biol. Chem., 277, 23294-23300.
    View publication on PubMed
Search for more literature for 2.7.1.138

Cloned(Commentary)

Cloned (Comment) Organism
transient expression in HEK293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
F-12509A 0.1 mM, 40-50% inhibition, sphingosine kinase inhibitor Homo sapiens
N,N-dimethylsphingosine 0.1 mM, 65% inhibition Homo sapiens
sphingosine 0.1 mM, 55% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 ATP pH 7.2, 30°C, recombinant ceramide kinase Homo sapiens
0.19
-
 ceramide pH 7.2, 30°C, recombinant ceramide kinase Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation above 0.0001 mM, maximal activation at 0.5 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TCT0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain mRNA level Homo sapiens
-
heart highest mRNA expression level Homo sapiens
-
kidney mRNA level Homo sapiens
-
liver mRNA level Homo sapiens
-
skeletal muscle mRNA level Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ceramide no activity sphingosine, D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine and phyto-sphingosine Homo sapiens ADP + ceramide 1-phosphate
-
 ?
ATP + hexadecanoylceramide C8 acyl chain, slightly higher activity than with natural ceramide Homo sapiens ADP + hexadecanoylceramide 1-phosphate
-
 ?
ATP + hexanoylceramide C6 acyl chain, similar activity than with natural ceramide, very low activity with C2-ceramide and C6-dihydroceramide, no activity with C2-dihydroceramide Homo sapiens ADP + hexanoylceramide 1-phosphate
-
 ?
ATP + octanoylceramide C8 acyl chain, higher activity than with natural ceramide Homo sapiens ADP + octanoylceramide 1-phosphate
-
 ?
ATP + octanoyldihydroceramide C8 acyl chain, slightly higher activity than with natural ceramide Homo sapiens ADP + octanoyldihydroceramide 1-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 approx. 25% of maximal activity at pH 6.0 and pH 8.0 Homo sapiens