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Literature summary for 2.7.1.137 extracted from
- Purification and characterization of phosphoinositide 3-kinase from rat liver (1990), J. Biol. Chem., 265, 19704-19711.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | - |
Rattus norvegicus |  |
| lysophosphatidic acid | - |
Rattus norvegicus | |
| phosphatidic acid | - |
Rattus norvegicus | |
| phosphatidylcholine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
phosphatidylinositol 4,5-bisphosphate | - |
Rattus norvegicus | |
| 0.009 | - |
phosphatidylinositol 4-phosphate | - |
Rattus norvegicus | |
| 0.03 | - |
ATP | with phosphatidylinositol 4-phosphate or phosphatidylinositol 4,5-bisphosphate as cosubstrate | Rattus norvegicus | |
| 0.06 | - |
phosphatidylinositol | - |
Rattus norvegicus | |
| 0.06 | - |
ATP | with phosphatidylinositol as cosubstrate | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect at physiological concentrations | Rattus norvegicus | |
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | maximal activation at 5 mM | Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation, about 10% of the activity with Mg2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
1 * 110000 + 1 * 85000, SDS-PAGE | Rattus norvegicus |
| 110000 | - |
1 * 110000 + 1 * 85000, SDS-PAGE | Rattus norvegicus |
| 190000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.058 | - |
production of phosphatidylinositol 3,4-diphosphate | Rattus norvegicus |
| 0.124 | - |
production of phosphatidylinositol 3-phosphate | Rattus norvegicus |
| 0.214 | - |
production of phosphatidylinositol 3,4,5-triphosphate | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Rattus norvegicus | ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| ATP + 1-phosphatidylinositol | - |
Rattus norvegicus | ADP + phosphatidylinositol 3-phosphate | - |
? | |
| ATP + phosphatidylinositol 4,5-bisphosphate | - |
Rattus norvegicus | ADP + phosphatidylinositol 3,4,5-trisphosphate | - |
? | |
| ATP + phosphatidylinositol 4-phosphate | - |
Rattus norvegicus | ADP + phosphatidylinositol 3,4-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 110000 + 1 * 85000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 80% of maximal activity, pH 9.0: about 40% of maximal activity | Rattus norvegicus |
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