Activating Compound | Comment | Organism | Structure |
---|---|---|---|
erythromycin | crude cell extracts from cells treated with erythromycin show increased activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoSO4 | 1 mM, 51% inhibition | Escherichia coli | |
CuSO4 | 1 mM, 52% inhibition | Escherichia coli | |
EDTA | 10 mM, 100% inhibition | Escherichia coli | |
FeSO4 | 1 mM, 66% inhibition | Escherichia coli | |
Iodine | 1 mM, 100% inhibition | Escherichia coli | |
additional information | 1 mM p-chloromercuribenzoic acid or urea are no inhibitors, MphR(A) is a repressor protein that binds to the promoter of the mph(A) gene and negatively regulates the enzyme expression | Escherichia coli | |
ZnSO4 | 1 mM, 93% inhibition | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Escherichia coli | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential | Escherichia coli | |
additional information | potassium chloride enhances reactivity, it is added in the reaction mixture at 0.06 M | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q47396 | macrolide 2'-phosphotransferase type I, high levels of activity with 14-member ring macrolides and extremely low levels with 16-member ring macrolides, accession code corresponds to nucleotide sequence for several proteins | - |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer pH 7, 0.06 M KCl, 0.01 M MgCl2, 0.006 M 2-mercaptoethanol, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + erythromycin | 87% activity with respect to oleandomycin | Escherichia coli | ADP + erythromycin-2'-O-phosphate | - |
? | |
ATP + josamycin | 1% activity with respect to oleandomycin | Escherichia coli | ? | - |
? | |
ATP + midecamycin | 3% activity with respect to oleandomycin | Escherichia coli | ? | - |
? | |
ATP + oleandomycin | - |
Escherichia coli | ADP + oleandomycin 2'-O-phosphate | - |
? | |
ATP + spectinomycin | 5% activity with respect to oleandomycin | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Escherichia coli |