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Literature summary for 2.7.1.13 extracted from

  • Sanchez-Moreno, I.; Trachtmann, N.; Ilhan, S.; Helaine, V.; Lemaire, M.; Guerard-Helaine, C.; Sprenger, G.A.
    2-Ketogluconate kinase from Cupriavidusnecator H16 purification, characterization, and exploration of its substrate specificity (2019), Molecules, 24, 2393 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Cupriavidus necator
gene kguK, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Cupriavidus necator

General Stability

General Stability Organism
the enzyme is stabilized by storage in an imidazole solution which is removed just before use Cupriavidus necator
the enzyme is unstable in its pure form Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics of the recombinant enzyme Cupriavidus necator
0.073
-
 ATP pH 8.0, temperature not specified in the publication Cupriavidus necator
0.35
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
1.4
-
 2-keto-3-deoxy-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
4
-
 2-keto-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
25
-
 2-keto-3-deoxy-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-dehydro-D-gluconate Cupriavidus necator
-
 ADP + 6-phospho-2-dehydro-D-gluconate
-
 r

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0K080
-
-
Cupriavidus necator Q0K080 i.e. Ralstonia eutropha strain H16
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and desalting gel filtration Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.7
-
 purified recombinant His-tagged enzyme, pH 8.0, 37°C Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-dehydro-D-gluconate
-
 Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gluconate
-
 ?
ATP + 2-dehydro-D-gluconate
-
 Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gluconate
-
 r
ATP + 2-dehydro-D-gluconate preferred substrate Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gluconate
-
 r
ATP + 2-dehydro-D-gulonate
-
 Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gulonate
-
 r
ATP + 2-keto-3-deoxy-D-gluconate
-
 Cupriavidus necator ADP + ?
-
 ?
ATP + 2-keto-3-deoxy-D-gulonate
-
 Cupriavidus necator ADP + ?
-
 ?
ATP + 2-keto-D-gulonate
-
 Cupriavidus necator ADP + ?
-
 ?
additional information the substrate specificity analysis reveals that three ketoacids (2-dehydro-3-deoxy-D-gluconate, 2-dehydro-D-gulonate, and 2-dehydro-3-deoxy-D-gulonate) with structures close to the natural substrate (2-dehydro-D-gluconate) are successfully phosphorylated at an efficiency lower than or comparable to 2-dehydrogluconate, as depicted by the measured kinetic constant values. Assay method optimization, overview Cupriavidus necator ?
-
-

Subunits

Subunits Comment Organism
? x * 35700, recombinant His-tagged enzyme, SDS-PAGE Cupriavidus necator

Synonyms

Synonyms Comment Organism
2-dehydrogluconokinase
-
 Cupriavidus necator
2-ketogluconate kinase
-
 Cupriavidus necator
KGUK
-
 Cupriavidus necator
KGUKCnec
-
 Cupriavidus necator
More see also EC 2.7.1.45 Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 2-keto-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.1
-
 2-keto-3-deoxy-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
5.71
-
 ATP pH 8.0, temperature not specified in the publication Cupriavidus necator
5.8
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
10.2
-
 2-keto-3-deoxy-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Cupriavidus necator

General Information

General Information Comment Organism
physiological function 2-ketogluconate kinase (KGUK) is involved in the glucose and 2-ketogluconate catabolism of several aerobic bacteria, but relatively few bacterial species are able to utilize 2-ketogluconate as the sole carbon source for growth and energy provision Cupriavidus necator

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.083
-
 2-keto-3-deoxy-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
0.246
-
 2-keto-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
7.37
-
 2-keto-3-deoxy-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
16.77
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
78.22
-
 ATP pH 8.0, temperature not specified in the publication Cupriavidus necator