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Literature summary for 2.7.1.127 extracted from

  • Koenig, S.; Moreau, C.; Dupont, G.; Scoumanne, A.; Erneux, C.
    Regulation of NGF-driven neurite outgrowth by Ins(1,4,5)P3 kinase is specifically associated with the two isoenzymes Itpka and Itpkb in a model of PC12 cells (2015), FEBS J., 282, 2553-2569.
    View publication on PubMed
Search for more literature for 2.7.1.127

Protein Variants

Protein Variants Comment Organism
additional information construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKA, generation of Itpka N-terminal F-actin binding domain (N66-GFP) and the N66(L34P)-GFP mutants. N-terminal deletion mutants are fully active Rattus norvegicus
additional information construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKB. N-terminal deletion mutants are fully active Rattus norvegicus
additional information construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKC Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P17105 gene Itpka
-
Rattus norvegicus P42335 gene Itpkb
-
Rattus norvegicus Q80ZG2 gene Itpkc
-

Source Tissue

Source Tissue Comment Organism Textmining
PC-12 cell
-
 Rattus norvegicus
-
pheochromocytoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ins(1,4,5)P3 kinase
-
 Rattus norvegicus
ITPKA
-
 Rattus norvegicus
Itpkb
-
 Rattus norvegicus
Itpkc
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

General Information

General Information Comment Organism
malfunction neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases. PC-12 cells overexpressing GFP-tagged kinase-dead mutants Itpka/b have shorter neurites than GFP control cells Rattus norvegicus
malfunction neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases. PC12 cells overexpressing GFP-tagged kinase-dead mutants Itpka/b have shorter neurites than GFP control cells Rattus norvegicus
additional information an F-actin binding site is located in the N-terminal part of isozyme Itpka. The catalytic activity is located at the C-terminal end, N-terminal deletion mutants are fully active Rattus norvegicus
additional information an F-actin binding site is located in the N-terminal part of isozyme Itpkb. The catalytic activity is located at the C-terminal end, N-terminal deletion mutants are fully active Rattus norvegicus
additional information neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases Rattus norvegicus
physiological function isozyme Itpka inhibits neurite outgrowth through both F-actin binding Rattus norvegicus
physiological function isozyme Itpkb inhibits neurite outgrowth through both F-actin binding Rattus norvegicus
physiological function isozyme Itpkc does not influence neurite length and neuron growth or F-actin binding Rattus norvegicus