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Literature summary for 2.7.1.127 extracted from

  • Shin, Y.S.; Choi, G.; Choi, K.Y.
    Overexpression, purification and characterization of inositol 1,4,5-triphosphate 3-kinase from rat brain (1995), Mol. Cells, 5, 348-353.
No PubMed abstract available
Search for more literature for 2.7.1.127

Cloned(Commentary)

Cloned (Comment) Organism
entire gene encoding IP3K is cloned and 130fold overexpressed in Escherichia coli BL21(DE3), when induced by isopropyl-beta-D-thiogalactoside Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 1D-myo-inositol 1,4,5-trisphosphate recombinant IP3K, in absence of Ca2+/calmodulin, 37°C Rattus norvegicus
0.0876
-
 ATP 37°C, recombinant IP3K Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol mainly Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.5fold activation by Ca2+/calmodulin Rattus norvegicus
Ca2+ activation by Ca2+/calmodulin Rattus norvegicus
Ca2+ not activated by free Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, recombinant IP3K overexpressed in Escherichia coli, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus IP3K regulates the intracellular level of the 2 second messengers Ins(1,4,5)P3 and Ins(1,3,4,5)P4 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol phosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
83fold Rattus norvegicus
recombinant IP3K overexpressed in Escherichia coli Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 recombinant IP3K overexpressed in Escherichia coli, in absence of calmodulin Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate IP3K regulates the intracellular level of the 2 second messengers Ins(1,4,5)P3 and Ins(1,3,4,5)P4 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate metabolism Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, recombinant IP3K overexpressed in Escherichia coli, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus
Calmodulin activation by Ca2+/calmodulin Rattus norvegicus
Calmodulin 1.5fold activation by Ca2+/calmodulin Rattus norvegicus