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Literature summary for 2.7.1.127 extracted from

  • Lee, S.Y.; Sim, S.S.; Kim, J.W.; Moon, K.H.; Kim, J.H.; Rhee, S.G.
    Purification and properties of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain. Susceptibility to calpain (1990), J. Biol. Chem., 265, 9434-9440.
    View publication on PubMed

General Stability

General Stability Organism
0.1% CHAPS or 0.1% Triton X-100 stabilizes, 0.5 mg/ml bovine serum albumin is less efficient in stabilizing Rattus norvegicus
bovine serum albumin stabilizes dilute enzyme solutions Rattus norvegicus
calpain inhibitors stabilize during purification Rattus norvegicus
ethylene glycol, 10%, KCl or sucrose, 0.25 M, does not stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme contains several motifs susceptible to a variety of proteases Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation by Ca2+/calmodulin Rattus norvegicus
Mg2+
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
1 * 53000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus involved in inositol polyphosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 0.1%, stabilizes Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
16100fold Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.9
-
pH 7.5, 37°C, in absence of calmodulin Rattus norvegicus
60
-
pH 7.5, 37°C, in presence of calmodulin Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C or 4°C, 0.1% CHAPS or Triton X-100, 4 days, less than 5% loss of activity Rattus norvegicus
-20°C, 4 days, 58% loss of activity Rattus norvegicus
4°C, 4 days, 20% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate involved in inositol polyphosphate metabolism Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rattus norvegicus
Calmodulin activation by Ca2+/calmodulin Rattus norvegicus