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Literature summary for 2.7.1.127 extracted from

  • Lin, A.; Wallace, R.W.; Barnes, S.
    Purification and properties of a human platelet inositol 1,4,5-trisphosphate 3-kinase (1993), Arch. Biochem. Biophys., 303, 412-420.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by Triton X-100 Homo sapiens

General Stability

General Stability Organism
enzyme is more stable in Tris buffer than in potassium phosphate buffer Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ at higher concentrations Homo sapiens
EGTA Ca2+/calmodulin-activated enzyme Homo sapiens
Protein kinase C phosphorylates and inactivates enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00076
-
1D-myo-inositol 1,4,5-trisphosphate pH 7.4, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
soluble
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at physiological concentrations Homo sapiens
Ca2+ activation by Ca2+/calmodulin Homo sapiens
Ca2+ mechanism of activation Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
1 * 36000, additional 53 kDa polypeptide with enzyme activity, SDS-PAGE Homo sapiens
58000
-
minor peaks with MW of 39 kDa and 32 kDa, HPLC gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens inositol 1,4,5-trisphosphate is a second messenger ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens key enzyme in inositol phosphate signaling pathway ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
943fold Homo sapiens
calmodulin-Sepharose affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
platelet
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.283
-
pH 7.4, 37°C Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris-HCl, pH 7.5, 1 mM EGTA, 3 mM MgCl2, 1 mM DTT, 10% v/v glycerol, several months, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol 1,4,5-trisphosphate is a second messenger Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate key enzyme in inositol phosphate signaling pathway Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, additional 53 kDa polypeptide with enzyme activity, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
in presence of Ca2+/calmodulin Homo sapiens
7.4
-
assay at Homo sapiens
7.6
-
in presence of EGTA Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.3 7.6 about half-maximal activity at pH 6.3 and 7.6 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
Calmodulin activation by Ca2+/calmodulin Homo sapiens
Calmodulin Ca2+/calmodulin-dependent, 10fold stimulation, reversed upon chelation of Ca2+ by EGTA Homo sapiens
Calmodulin mechanism of activation Homo sapiens