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Literature summary for 2.7.1.113 extracted from

  • Wang, L.; Eriksson, S.
    Mitochondrial deoxyguanosine kinase mutations and mitochondrial DNA depletion syndrome (2003), FEBS Lett., 554, 319-322.
    View publication on PubMed
Search for more literature for 2.7.1.113

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
E227K site-directed mutagenesis, mutation is naturally found in patients, unaltered Km but very low Vmax for all substrates compared to the wild-type enzyme Homo sapiens
additional information C-terminally truncated mutants are catalytically inactive, naturally occuring genetic defects/mutations, overview Homo sapiens
R142K site-directed mutagenesis, mutation is naturally found in patients, low activity with dG, no activity with dA compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 2'-deoxyguanosine pH 7.6, recombinant wild-type enzyme Homo sapiens
0.0052
-
 2'-deoxyguanosine pH 7.6, recombinant mutant E227K Homo sapiens
0.0085
-
 ATP pH 7.6, recombinant wild-type enzyme, with 2'-deoxyguanosine Homo sapiens
0.014
-
 ATP pH 7.6, recombinant mutant E227K, with 2'-deoxyguanosine Homo sapiens
0.0974
-
 ATP pH 7.6, recombinant mutant R142K, with 2'-deoxyguanosine Homo sapiens
0.123
-
 2'-deoxyguanosine pH 7.6, recombinant mutant R142K Homo sapiens
0.242
-
 ATP pH 7.6, recombinant mutant E227K, with 2'-deoxyadenosine Homo sapiens
0.269
-
 ATP pH 7.6, recombinant wild-type enzyme, with 2'-deoxyadenosine Homo sapiens
0.414
-
 2'-deoxyadenosine pH 7.6, recombinant mutant E227K Homo sapiens
0.467
-
 2'-deoxyadenosine pH 7.6, recombinant wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxyadenosine Homo sapiens
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine Homo sapiens
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
additional information Homo sapiens th enzyme catalyzes the initial phosphorylation of purine deoxynucleosides, enzyme deficiency leads to severe mitochondrial DNA depletion diseases ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + deoxyguanosine = ADP + dGMP active site residues are Glu70, Lys142, and Arg142 Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 catalytic efficiencies of wild-type and muztant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
additional information th enzyme catalyzes the initial phosphorylation of purine deoxynucleosides, enzyme deficiency leads to severe mitochondrial DNA depletion diseases Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
DGK
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens