Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L93A | single-point mutant, constructed to destabilize the interface of isoform Pfk2. The mutant is an inactive monomer at protein concentrations below 30 microM. Active dimer formation can be induced by increasing the protein concentration and by addition of its substrate fructose-6-phosphate. Unfolding occurs noncooperatively and the isolated subunit is partially unstructured and marginally stable | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P06999 | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | the isolated subunit has overall higher solvent accessibility than the native dimer, with the exception of residues 240-309, corresponding to most of the beta-meander module. The hydrophobic core of the isolated monomer of Pfk2 is solvent-penetrated in native conditions and the beta-meander module is not affected by monomerizing mutations | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent 6-phosphofructokinase isozyme 2 | - |
Escherichia coli |
pfkB | - |
Escherichia coli |