Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | - |
Oryctolagus cuniculus | |
D-fructose 2,6-bisphosphate | - |
Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in a pfk-deficient Escherichia coli strain DF1020DE3 | Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 16% (w/v) PEG 400, 0.1 M MgSO4, and 0.1 M acetate buffer (pH 5.3) | Oryctolagus cuniculus |
hanging drop vapor diffusion method, using 6-10% (w/v) PEG 4000 and 0.2 mM sodium acetate in 0.1 M 4-morpholineethanesulfonic acid buffer (pH 6.0) | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P00511 | - |
- |
Saccharomyces cerevisiae | P16862 | - |
- |
Purification (Comment) | Organism |
---|---|
Cibacron Blue 3GA gel filtration, DE-52 column chromatography and ATP-N6-agarose column chromatography | Oryctolagus cuniculus |
PEG 6000 precipitation at 3.5% and 14% (w/v) and Cibacron Blue F3G-A Sephadex G 100 gel filtration | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterooctamer | x-ray crystallography | Saccharomyces cerevisiae |
heterotetramer | x-ray crystallography | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
PFK | - |
Oryctolagus cuniculus |
PFK | - |
Saccharomyces cerevisiae |
phosphofructokinase 1 | - |
Oryctolagus cuniculus |
phosphofructokinase 1 | - |
Saccharomyces cerevisiae |