Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | the active 49 kDa PFK1 fragment is activated to a higher level by D-fructose 2,6-bisphosphate with respect to the native enzyme, a significant boost in the enzyme activity of the fragment is registered at 1 mM ATP after the introduction of 0.004 mM of D-fructose-2,6-bisphosphate | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | in contrast to the native PFK1 enzyme, the 49 kDa PFK1 fragment is highly sensitive to ATP inhibition (5fold lower activity at the 1 mM ATP than at the 0.1 mM concentration) | Aspergillus niger | |
citrate | when 5 mM of citrate is added a moderate reduction for about 1020% is recorded in the homogenate of TE22 and TE23 strain, while in A158 strain much stronger reduction of PFK1 specific activity, for approximately 40% is observed | Aspergillus niger | |
additional information | the active 49 kDa PFK1 fragment is not inhibited by citrate | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
native enzyme, gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | P78985 | strains A158, TE22, and TE23 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | in Aspergillus niger cells spontaneous posttranslational modification of 6-phosphofructo-1-kinase occurs in a two step process. The native enzyme (85 kDa) is first cleaved to an inactive fragment (49 kDa) that regains its activity after phosphorylation of the protein, the shorter fragment exhibits changed kinetics, such as resistance to citrate inhibition | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Aspergillus niger | ADP + D-fructose 1,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-1-kinase | - |
Aspergillus niger |
PFK1 | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Aspergillus niger |