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Literature summary for 2.7.1.11 extracted from

  • Capuder, M.; Solar, T.; Bencina, M.; Legisa, M.
    Highly active, citrate inhibition resistant form of Aspergillus niger 6-phosphofructo-1-kinase encoded by a modified pfkA gene (2009), J. Biotechnol., 144, 51-57.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate the active 49 kDa PFK1 fragment is activated to a higher level by D-fructose 2,6-bisphosphate with respect to the native enzyme, a significant boost in the enzyme activity of the fragment is registered at 1 mM ATP after the introduction of 0.004 mM of D-fructose-2,6-bisphosphate Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
ATP in contrast to the native PFK1 enzyme, the 49 kDa PFK1 fragment is highly sensitive to ATP inhibition (5fold lower activity at the 1 mM ATP than at the 0.1 mM concentration) Aspergillus niger
citrate when 5 mM of citrate is added a moderate reduction for about 10–20% is recorded in the homogenate of TE22 and TE23 strain, while in A158 strain much stronger reduction of PFK1 specific activity, for approximately 40% is observed Aspergillus niger
additional information the active 49 kDa PFK1 fragment is not inhibited by citrate Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
native enzyme, gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger P78985 strains A158, TE22, and TE23
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein in Aspergillus niger cells spontaneous posttranslational modification of 6-phosphofructo-1-kinase occurs in a two step process. The native enzyme (85 kDa) is first cleaved to an inactive fragment (49 kDa) that regains its activity after phosphorylation of the protein, the shorter fragment exhibits changed kinetics, such as resistance to citrate inhibition Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Aspergillus niger ADP + D-fructose 1,6-bisphosphate
-
?

Synonyms

Synonyms Comment Organism
6-phosphofructo-1-kinase
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Aspergillus niger
PFK1
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Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
ATP
-
Aspergillus niger