Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-diphosphoglycerate | 0.385 mM and 0.005 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition | Musa acuminata | |
2-phosphoglycerate | 0.032 mM and 0.004 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition | Musa acuminata | |
ADP | 1.9 mM and 0.0006 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition | Musa acuminata | |
phosphoenolpyruvate | 0.0023 mM and 0.0009 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition | Musa acuminata | |
Phosphoglycolate | 0.006 mM and 0.003 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition | Musa acuminata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
ATP | pH 7.3, 25°C, PFK II | Musa acuminata | |
0.03 | - |
CTP | pH 7.3, 25°C, PFK II | Musa acuminata | |
0.032 | - |
UTP | pH 7.3, 25°C, PFK II | Musa acuminata | |
0.034 | - |
ATP | pH 8.0, 25°C, PFK I | Musa acuminata | |
0.035 | - |
ITP | pH 7.3, 25°C, PFK II | Musa acuminata | |
0.038 | - |
GTP | pH 7.3, 25°C, PFK II | Musa acuminata | |
0.055 | - |
ATP | - |
Musa acuminata | |
0.055 | - |
UTP | pH 8.0, 25°C, PFK I | Musa acuminata | |
0.082 | - |
ITP | pH 8.0, 25°C, PFK I | Musa acuminata | |
0.099 | - |
CTP | pH 8.0, 25°C, PFK I | Musa acuminata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
PFK II, gel filtration | Musa acuminata |
210000 | - |
PFK I, gel filtration | Musa acuminata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Musa acuminata | - |
L. cv. Cavendish, banana, isoenzymes PFK I and PFK II | - |
Purification (Comment) | Organism |
---|---|
PFK I and PFK II, butyl-Sepharose, DEAE-Fractogel, Superose 6 | Musa acuminata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | ripened fruit | Musa acuminata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.5 | - |
PFK II | Musa acuminata |
6 | - |
PFK I | Musa acuminata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Musa acuminata | ADP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | - |
Musa acuminata | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Musa acuminata | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | - |
Musa acuminata | IDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Musa acuminata | UDP + D-fructose 1,6-bisphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
PFK II | Musa acuminata |
8 | - |
PFK I | Musa acuminata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8.7 | PFK II, approx. 30% of maximal activity at pH 6.5, approx. 10% of maximal activity at pH 8.6 | Musa acuminata |
6.5 | 9 | PFK I, approx. 10% of maximal activity at pH 6.5, approx. 60% of maximal activity at pH 9.0 | Musa acuminata |