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Literature summary for 2.7.1.11 extracted from

  • Alves, A.M.; Euverink, G.J.; Santos, H.; Dijkhuizen, L.
    Different physiological roles of ATP- and PP(i)-dependent phosphofructokinase isoenzymes in the methylotrophic actinomycete Amycolatopsis methanolica (2001), J. Bacteriol., 183, 7231-7240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Amycolatopsis methanolica

Inhibitors

Inhibitors Comment Organism Structure
AMP 1 mM and 4 mM, 20% and 40% inhibition in the presence of 5 mM fructose 6-phosphate and Mg2+ Amycolatopsis methanolica
diphosphate 1 mM and 4 mM, 20% and 50% inhibition in the presence of 5 mM fructose 6-phosphate and 5 mM Mg2+ Amycolatopsis methanolica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
Mg2+ pH 7.5, 37°C Amycolatopsis methanolica
0.6 0.9 ATP pH 7.5, 37°C Amycolatopsis methanolica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Amycolatopsis methanolica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48500
-
x * 48500, deduced from nucleotide sequence Amycolatopsis methanolica
50000
-
2 * 50000, SDS-PAGE Amycolatopsis methanolica
110000
-
gel filtration Amycolatopsis methanolica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Amycolatopsis methanolica
-
ADP + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Amycolatopsis methanolica Q8VU09 methylotrophic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Levafix blue, Resource Q, Phenyl-Sepharose Amycolatopsis methanolica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
-
Amycolatopsis methanolica

Storage Stability

Storage Stability Organism
-20°C, 40% glycerol, 6 months, no loss of activity Amycolatopsis methanolica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Amycolatopsis methanolica ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate absolutely specific for fructose 6-phosphate Amycolatopsis methanolica ADP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 6-phosphate 100% of activity with ATP Amycolatopsis methanolica GDP + D-fructose 1,6-bisphosphate
-
?
UTP + D-fructose 6-phosphate 40% of activity with ATP Amycolatopsis methanolica UDP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
? x * 48500, deduced from nucleotide sequence Amycolatopsis methanolica
dimer 2 * 50000, SDS-PAGE Amycolatopsis methanolica