Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Amycolatopsis methanolica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 1 mM and 4 mM, 20% and 40% inhibition in the presence of 5 mM fructose 6-phosphate and Mg2+ | Amycolatopsis methanolica | |
diphosphate | 1 mM and 4 mM, 20% and 50% inhibition in the presence of 5 mM fructose 6-phosphate and 5 mM Mg2+ | Amycolatopsis methanolica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
Mg2+ | pH 7.5, 37°C | Amycolatopsis methanolica | |
0.6 | 0.9 | ATP | pH 7.5, 37°C | Amycolatopsis methanolica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Amycolatopsis methanolica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48500 | - |
x * 48500, deduced from nucleotide sequence | Amycolatopsis methanolica |
50000 | - |
2 * 50000, SDS-PAGE | Amycolatopsis methanolica |
110000 | - |
gel filtration | Amycolatopsis methanolica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Amycolatopsis methanolica | - |
ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis methanolica | Q8VU09 | methylotrophic bacterium | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Levafix blue, Resource Q, Phenyl-Sepharose | Amycolatopsis methanolica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
- |
Amycolatopsis methanolica |
Storage Stability | Organism |
---|---|
-20°C, 40% glycerol, 6 months, no loss of activity | Amycolatopsis methanolica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Amycolatopsis methanolica | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | absolutely specific for fructose 6-phosphate | Amycolatopsis methanolica | ADP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | 100% of activity with ATP | Amycolatopsis methanolica | GDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | 40% of activity with ATP | Amycolatopsis methanolica | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48500, deduced from nucleotide sequence | Amycolatopsis methanolica |
dimer | 2 * 50000, SDS-PAGE | Amycolatopsis methanolica |