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Literature summary for 2.7.1.11 extracted from

  • Brooks, S.P.J.; Storey, K.B.
    Purification of phosphofructokinase using transition-state analogue affinity chromatography (1988), J. Chromatogr., 455, 291-296.
    View publication on PubMed

General Stability

General Stability Organism
NaF, EDTA and PMSF stabilize during purification Trachemys scripta

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Trachemys scripta
Mg2+ MgATP is the active substrate Trachemys scripta

Organism

Organism UniProt Comment Textmining
Trachemys scripta
-
freshwater turtle
-

Purification (Commentary)

Purification (Comment) Organism
3 isozymes from heart, brain or muscle, DEAE-Sepharose, ADP-agarose Trachemys scripta

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Trachemys scripta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
95
-
brain PFK Trachemys scripta
106.3
-
muscle PFK Trachemys scripta
150
-
heart PFK Trachemys scripta

Storage Stability

Storage Stability Organism
4°C, 40 mM potassium phosphate, pH 7.0, 10 mM beta-mercaptoethanol, 0.1 mM EDTA, 2% glycerol, 6 mM fructose 6-phosphate, quick loss of activity Trachemys scripta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Trachemys scripta ADP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Trachemys scripta