General Stability | Organism |
---|---|
NaF, EDTA and PMSF stabilize during purification | Trachemys scripta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Trachemys scripta | |
Mg2+ | MgATP is the active substrate | Trachemys scripta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trachemys scripta | - |
freshwater turtle | - |
Purification (Comment) | Organism |
---|---|
3 isozymes from heart, brain or muscle, DEAE-Sepharose, ADP-agarose | Trachemys scripta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Trachemys scripta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
95 | - |
brain PFK | Trachemys scripta |
106.3 | - |
muscle PFK | Trachemys scripta |
150 | - |
heart PFK | Trachemys scripta |
Storage Stability | Organism |
---|---|
4°C, 40 mM potassium phosphate, pH 7.0, 10 mM beta-mercaptoethanol, 0.1 mM EDTA, 2% glycerol, 6 mM fructose 6-phosphate, quick loss of activity | Trachemys scripta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Trachemys scripta | ADP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Trachemys scripta |