Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | - |
Bacillus licheniformis |
General Stability | Organism |
---|---|
D-fructose 6-phosphate stabilizes, less effective is KCl or phosphoenolpyruvate, only slightly stabilized by ATP or ATP/Mg2+ | Bacillus sp. (in: Bacteria) |
D-fructose 6-phosphate stabilizes, less effective is KCl or phosphoenolpyruvate, only slightly stabilized by ATP or ATP/Mg2+ | Bacillus licheniformis |
dilution inactivates | Bacillus sp. (in: Bacteria) |
dilution inactivates | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | competitive vs. Mg2+ | Bacillus licheniformis | |
citrate | - |
Bacillus licheniformis | |
diphosphate | inactivates under assay conditions | Bacillus licheniformis | |
phosphoenolpyruvate | effective inhibition at 0.005-0.02 mM, fructose 6-phosphate relieves inhibition; most potent inhibitor | Bacillus licheniformis | |
phosphoenolpyruvate | most potent inhibitor | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-fructose 6-phosphate | - |
Bacillus sp. (in: Bacteria) | |
0.05 | - |
D-fructose 6-phosphate | pH 6.8, 30°C | Bacillus licheniformis | |
0.07 | - |
ATP | pH 6.8, 30°C | Bacillus licheniformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bacillus sp. (in: Bacteria) | 5829 | - |
cytosol | - |
Bacillus licheniformis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Bacillus sp. (in: Bacteria) | |
K+ | - |
Bacillus licheniformis | |
Mg2+ | required for activity | Bacillus sp. (in: Bacteria) | |
Mg2+ | required for activity | Bacillus licheniformis | |
additional information | not activated by Li+ | Bacillus sp. (in: Bacteria) | |
additional information | not activated by Li+ | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
- |
Bacillus sp. (in: Bacteria) |
135000 | - |
sucrose density gradient centrifugation | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
e.g. Bacillus cereus, Bacillus megaterium, Bacillus mycoides, Bacillus subtilis 168M | - |
Purification (Comment) | Organism |
---|---|
partial | Bacillus sp. (in: Bacteria) |
partial | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.6 | - |
- |
Bacillus licheniformis |
Storage Stability | Organism |
---|---|
-20°C, in phosphate buffer, 50% glycerol, 0.3% loss of activity per day | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Bacillus sp. (in: Bacteria) | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Bacillus licheniformis | ADP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus sp. (in: Bacteria) |
30 | - |
assay at | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
- |
Bacillus sp. (in: Bacteria) |
- |
- |
rapid inactivation in the presence of Mg2+, phosphoenolpyruvate and ATP, incubation at 30°C reverses activity, inactivation is probably due to dissociation of a dimer into monomers | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.7 | - |
Bacillus licheniformis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Ca2+ | pH 6.8, 30°C | Bacillus licheniformis |