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Literature summary for 2.7.1.11 extracted from

  • Cronin, C.N.; Tipton, K.F.
    Purification and regulatory properties of phosphofructokinase from Trypanosoma (Trypanozoon) brucei brucei (1985), Biochem. J., 227, 113-124.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
ADP 1.8 mM, half-maximal activation at fructose 6-phosphate concentrations below 0.5 mM Trypanosoma brucei
AMP 0.64 mM, half-maximal activation at fructose 6-phosphate concentrations below 0.5 mM Trypanosoma brucei
cAMP 0.4 mM, half-maximal activation at fructose 6-phosphate concentrations below 0.5 mM Trypanosoma brucei

General Stability

General Stability Organism
AMP, ADP, fructose 6-phosphate or antipain stabilizes during purification Trypanosoma brucei
concentration by ultrafiltration on Amicon PM-10 membrane results in appreciable loss of activity, concentration in collodium bags allows higher protein concentration to be obtained without loss of activity Trypanosoma brucei
dilution leads to enzyme dissociation Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
ADP kinetics Trypanosoma brucei
AMP kinetics Trypanosoma brucei
citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Trypanosoma brucei
additional information not inhibited by fructose 1,6-bisphosphate; not inhibited by fructose 2,6-bisphosphate; not inhibited by phosphate Trypanosoma brucei
phosphoenolpyruvate inhibition kinetic Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Trypanosoma brucei
0.013
-
 ATP pH 6.7, 37°C, at 0.25 mM fructose 6-phosphate Trypanosoma brucei
0.017
-
 ATP pH 6.7, 37°C, at 0.4 mM fructose 6-phosphate Trypanosoma brucei
0.023
-
 ATP pH 6.7, 37°C, at 0.75 mM fructose 6-phosphate Trypanosoma brucei
0.026
-
 ATP pH 6.7, 37°C, at 2.0 mM fructose 6-phosphate Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
 Trypanosoma brucei 20015
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 4 * 49000, SDS-PAGE Trypanosoma brucei
220000
-
 glycerol density gradient centrifugation Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, ATP-agarose, purification at pH 8.0 in the presence of antipain Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140 180
-
 Trypanosoma brucei

Storage Stability

Storage Stability Organism
-20°C, concentrated enzyme solution in 50 mM Tris-HCl buffer, pH 7.8, 60% glycerol, 1 mM EDTA, 10 mM dithiothreitol, 13% loss of activity per month Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Trypanosoma brucei ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 49000, SDS-PAGE Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei