Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | 25% activation at 5 mM, inhibition above | Monoraphidium minutum |
General Stability | Organism |
---|---|
at least 15 mM phosphate required to stabilize PFK during purification, presence of 10% glycerol helps to stabilize | Monoraphidium minutum |
largest molecular weight-form of purified enzyme is the most stable form | Monoraphidium minutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 2 mM, 92% inhibition | Monoraphidium minutum | |
3-phosphoglycerate | 2 mM, 86% inhibition; inhibition kinetics | Monoraphidium minutum | |
ATP | above 0.2 mM, inhibition is alleviated by Mg2+; free form | Monoraphidium minutum | |
citrate | 2 mM, complete inhibition | Monoraphidium minutum | |
malate | 2 mM, 92% inhibition | Monoraphidium minutum | |
additional information | not inhibited by 2-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by glutamine, glutamate | Monoraphidium minutum | |
phosphoenolpyruvate | 2 mM, complete inhibition; inhibition kinetic | Monoraphidium minutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Monoraphidium minutum | |
0.009 | - |
ATP | pH 7.25, 25°C | Monoraphidium minutum | |
0.01 | - |
ATP | - |
Monoraphidium minutum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Monoraphidium minutum | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km: 0.5 mM | Monoraphidium minutum | |
Mg2+ | required for activity | Monoraphidium minutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
4 * 70000, SDS-PAGE, smallest catalytically active form | Monoraphidium minutum |
280000 | - |
gel filtration, smallest catalytically active form, tends to aggregate to different active forms | Monoraphidium minutum |
6000000 | - |
gel filtration, largest catalytically active form, tends to aggregate to different active forms | Monoraphidium minutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Monoraphidium minutum | - |
green alga | - |
Purification (Comment) | Organism |
---|---|
no isozymes | Monoraphidium minutum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
122 | 180 | - |
Monoraphidium minutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Monoraphidium minutum | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 70000, SDS-PAGE, smallest catalytically active form | Monoraphidium minutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Monoraphidium minutum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | approx. half-maximal activity at pH 6.5 and pH 8.2 | Monoraphidium minutum |