Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | 1 mM, strong activtion of plastid PFK at pH-values below 7.0 | Ricinus communis | |
phosphate | cytosolic isozyme: slight activation | Ricinus communis |
General Stability | Organism |
---|---|
cytosolic enzyme extremely unstable upon purification, substrates, metabolites, ethylene glycol or SH-compounds do not stabilize | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | substrate inhibition | Ricinus communis | |
additional information | cytosolic PFK is not inhibited by ATP and SO42- | Ricinus communis | |
SO42- | 2 mM, 50% inhibition at pH 7.2, 5 mM complete inhibition | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic and plastidic isoenzyme | Ricinus communis | 5829 | - |
plastid | - |
Ricinus communis | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
175000 | - |
plastid PFK, gel filtration | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
plastid PFK, acetone powder, DEAE-Sephacel, AMP-agarose, DEAE-Sepharose | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating | Ricinus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
plastid PFK | Ricinus communis |
Storage Stability | Organism |
---|---|
ATP, 1 mM, stabilizes during storage for longer periods | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Ricinus communis | ADP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.3 | cytosolic isozyme | Ricinus communis |
7.6 | 8 | plastid isoenzyme | Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9 | approx. half-maximal activity at pH 6.8 and 9, plastid isoenzyme | Ricinus communis |