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Literature summary for 2.7.1.11 extracted from

  • Garland, W.J.; Dennis, D.T.
    Plastid and cytosolic phosphofructokinase from the developing endosperm of Ricinus communis. I. Separation, purification, and initial characterization of the isozymes (1980), Arch. Biochem. Biophys., 204, 302-309.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate 1 mM, strong activtion of plastid PFK at pH-values below 7.0 Ricinus communis
phosphate cytosolic isozyme: slight activation Ricinus communis

General Stability

General Stability Organism
cytosolic enzyme extremely unstable upon purification, substrates, metabolites, ethylene glycol or SH-compounds do not stabilize Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
ATP substrate inhibition Ricinus communis
additional information cytosolic PFK is not inhibited by ATP and SO42- Ricinus communis
SO42- 2 mM, 50% inhibition at pH 7.2, 5 mM complete inhibition Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic and plastidic isoenzyme Ricinus communis 5829
-
plastid
-
Ricinus communis 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
plastid PFK, gel filtration Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
plastid PFK, acetone powder, DEAE-Sephacel, AMP-agarose, DEAE-Sepharose Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21
-
plastid PFK Ricinus communis

Storage Stability

Storage Stability Organism
ATP, 1 mM, stabilizes during storage for longer periods Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Ricinus communis ADP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 8.3 cytosolic isozyme Ricinus communis
7.6 8 plastid isoenzyme Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
6.8 9 approx. half-maximal activity at pH 6.8 and 9, plastid isoenzyme Ricinus communis