Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | 10 mM, 60% inhibition | Musa acuminata | |
Dithionitrobenzoic acid | 0.06 mM, 80% inhibition of PFK III, reversed by 2-mercaptoethanol, dithiothreitol or reduced glutathione | Musa acuminata | |
additional information | not inhibited by AMP; not inhibited by cAMP; not inhibited by phosphate | Musa acuminata | |
p-Chloromercurisulfonic acid | 0.025 mM, 80% inhibition of PFK III, reversed by 2-mercaptoethanol, dithiothreitol or reduced glutathione | Musa acuminata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Musa acuminata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Musa acuminata | |
Mg2+ | substrate activator and enzyme protein modulator | Musa acuminata | |
Mg2+ | MgATP is the active substrate | Musa acuminata | |
Mn2+ | activation | Musa acuminata | |
Mn2+ | 60% of activity with Mg2+, above 3 mM | Musa acuminata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Musa acuminata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
monomeric form PFK-III, ammonium sulfate, heat treatment, Sepharose 6B, DEAE-sephacel | Musa acuminata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate | sequential random mechanism | Musa acuminata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Musa acuminata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.6 | - |
PFK III | Musa acuminata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | no activity with glucose 6-phosphate | Musa acuminata | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | transfers gamma-phosphate of ATP to C-1-hydroxyl of D-(-)fructose 6-phosphate, highly specific for sugar substrate | Musa acuminata | ADP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | as effective as ATP | Musa acuminata | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | almost as effective as ATP | Musa acuminata | GDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | as effective as ATP | Musa acuminata | UDP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
complete inactivation of PFK III after approx. 30 sec, ATP stabilizes, 30% loss of activity after 6 min in the presence of 1 mM ATP | Musa acuminata |