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Literature summary for 2.7.1.11 extracted from
- Properties of ATP-dependent phosphofructokinase from endosperm of developing wheat (Triticum aestivum L.) grains (1992), J. Plant Biochem. Biotechnol., 1, 45-48.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Triticum aestivum |  |
| ADP | kinetics | Triticum aestivum | |
| citrate | - |
Triticum aestivum | |
| D-fructose 1,6-bisphosphate | product inhibition | Triticum aestivum | |
| phosphoenolpyruvate | - |
Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
ATP | pH 7.9 | Triticum aestivum | |
| 1.43 | - |
D-fructose 6-phosphate | pH 7.9 | Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 182000 | - |
- |
Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, Sepharose CL-6B, Sephadex G-200 | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | transfers gamma-phosphate of ATP to C-1-hydroxyl of D-(-)fructose 6-phosphate, highly specific for sugar substrate | Triticum aestivum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Triticum aestivum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
phosphoenolpyruvate | pH 7.9 | Triticum aestivum | |
| 1.75 | - |
ADP | pH 7.9 | Triticum aestivum | |
| 2.1 | - |
3-phosphoglycerate | pH 7.9 | Triticum aestivum | |
| 2.4 | - |
citrate | pH 7.9 | Triticum aestivum | |
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