Cloned (Comment) | Organism |
---|---|
gene At3g45040 or AtDOK1, DNA and amino acid sequence determination and analysis, a cytidylyltransferase motif-containing gene, phylogenetic tree. The gene complements defects in the growth and N-linked glycosylation of the Saccharomyces cerevisiae sec59 mutant, deficient in dolichol kinase Sec59p | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of two independent lines of T-DNA-tagged AtDOK1 mutants, designated dok1-1 (CS822420; SAIL_529_E06) and dok1-2 (CS856101; WiscDsLox443D6), with T-DNA insertions in exons 8 and 13, respectively. Mutant dok1-1/+ and wild-type seed morphologies show no significant differences, and dok1-1/+ and the wild type did not differ in seed germination rates | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + dolichol | Arabidopsis thaliana | - |
CDP + dolichyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | F4J4C8 | gene At3g45040 or DOK1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
anther | DOK1 is highly expressed in tapetum cells and microspores during early anther development | Arabidopsis thaliana | - |
microspore | - |
Arabidopsis thaliana | - |
additional information | gene AtDOK1 is expressed in specific cell types involved in meristematic activities, analysis of tissue-specific expression of DOK1 by histochemical GUS staining, overview | Arabidopsis thaliana | - |
plant ovule | high expression level | Arabidopsis thaliana | - |
root | root tip of germinating seedlings, and in the founder cells where lateral root growth is initiated | Arabidopsis thaliana | - |
seedling | root tip of germinating seedlings | Arabidopsis thaliana | - |
shoot apex | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
tapetum | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + dolichol | - |
Arabidopsis thaliana | CDP + dolichyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DOK1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CTP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the heterozygous T-DNA-tagged AtDOK1 mutant, dok1-1 and dok1-2, plants show developmental defects in male and female gametophytes, including an aberrant pollen structure, low pollen viability, and short siliques. Additionally, the mutations has incomplete penetrance. Mutation of dok-1 may affect development of siliques but not seed maturation or germination in Arabidopsis thaliana. Male gametophytic defect in dok1 heterozygous plants, overview | Arabidopsis thaliana |
physiological function | the enzyme is required for reproductive processes in Arabidopsis thaliana | Arabidopsis thaliana |