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Literature summary for 2.7.1.108 extracted from

  • Ravi, K.; Rip, J.W.; Carroll, K.K.
    Characterization of dolichol kinase from soybean microsomes (1986), Biochim. Biophys. Acta, 875, 618-625.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100
-
Glycine max

Inhibitors

Inhibitors Comment Organism Structure
CDP
-
Glycine max
CTP
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
dolichol pH 7.4, 37°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Glycine max 16020
-
microsome
-
Glycine max
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ low level of stimulation noted, 80% of maximal activity is observed in absence of divalent kcations Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + dolichol Glycine max
-
CDP + dolichyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + dolichol
-
Glycine max CDP + dolichyl phosphate
-
?
additional information CTP or other phosphate group donors like ATP, GTP or UTP are not required for maximal activity Glycine max ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 second optimum at pH 9.5 Glycine max
9.5
-
second optimum at pH 7.0-8.0 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
4 11 no second optimum with Tris-HCl, activity increases fairly linearly between pH 8.0 and 10.0, activity decreases about 25% between pH 9.5 and 10.0 in Chaps buffer Glycine max