BRENDA - Enzyme Database
show all sequences of 2.7.1.108

Properties of brain dolichol kinase activity solubilized with a zwitterionic detergent

Sumbilla, C.; Waechter, C.J.; Arch. Biochem. Biophys. 238, 75-82 (1985)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
(R)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
(S)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
phosphatidylcholine
stimulated by after dialysis
Bos taurus
phosphatidylethanolamine
stimulated by after dialysis
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
CDP
-
Bos taurus
dCDP
-
Bos taurus
EDTA
-
Bos taurus
additional information
not inhibited by CMP, ADP, GDP or UDP
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
CTP
pH 7.2, 37°C
Bos taurus
0.0091
-
dCTP
pH 7.2, 37°C
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bos taurus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Bos taurus
Zn2+
markedly stimulated by
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + dolichol
Bos taurus
-
CDP + dolichyl phosphate
-
Bos taurus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
calf
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Bos taurus
-
Storage Stability
Storage Stability
Organism
-17°C, 65% of original activity retains when stored for 6 weeks
Bos taurus
4°C, no detectable loss of activity when solubilized extract is kept for 24 h
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + dolichol
-
640396
Bos taurus
CDP + dolichyl phosphate
-
640396
Bos taurus
?
dCTP + dolichol
-
640396
Bos taurus
dCDP + dolichyl phosphate
-
640396
Bos taurus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Bos taurus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.121
-
CDP
pH 7.2, 37°C
Bos taurus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
(R)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
(S)-dolichol (C95)
markedly stimulated by addition, Km 0.009 mM
Bos taurus
phosphatidylcholine
stimulated by after dialysis
Bos taurus
phosphatidylethanolamine
stimulated by after dialysis
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CDP
-
Bos taurus
dCDP
-
Bos taurus
EDTA
-
Bos taurus
additional information
not inhibited by CMP, ADP, GDP or UDP
Bos taurus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.121
-
CDP
pH 7.2, 37°C
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
CTP
pH 7.2, 37°C
Bos taurus
0.0091
-
dCTP
pH 7.2, 37°C
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Bos taurus
Zn2+
markedly stimulated by
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + dolichol
Bos taurus
-
CDP + dolichyl phosphate
-
Bos taurus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Bos taurus
-
Storage Stability (protein specific)
Storage Stability
Organism
-17°C, 65% of original activity retains when stored for 6 weeks
Bos taurus
4°C, no detectable loss of activity when solubilized extract is kept for 24 h
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + dolichol
-
640396
Bos taurus
CDP + dolichyl phosphate
-
640396
Bos taurus
?
dCTP + dolichol
-
640396
Bos taurus
dCDP + dolichyl phosphate
-
640396
Bos taurus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Bos taurus
Other publictions for EC 2.7.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739314
Nakamura
Arabidopsis DOK1 encodes a fun ...
Arabidopsis thaliana
Plant J.
81
292-303
2015
-
-
1
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739079
Lieu
Severe, fatal multisystem mani ...
Homo sapiens
Mol. Genet. Metab.
110
484-489
2013
-
-
1
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
723805
Juchimiuk
Defect in dolichol-dependent g ...
Saccharomyces cerevisiae
Yeast
27
637-645
2010
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
696448
Denecke
Hypoglycosylation due to dolic ...
Homo sapiens
Biochim. Biophys. Acta
1792
888-895
2009
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
687738
Han
Characterization of the yeast ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
20443-20453
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687781
Rush
Recycling of dolichyl monophos ...
Bos taurus
J. Biol. Chem.
283
4087-4093
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671201
Kranz
A defect in dolichol phosphate ...
Homo sapiens
Am. J. Hum. Genet.
80
433-440
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677274
Orlowski
Dissecting the role of dolicho ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae SF402-4D
Yeast
24
239-252
2007
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674673
Shridas
Human dolichol kinase, a polyt ...
Homo sapiens
J. Biol. Chem.
281
31696-31704
2006
-
-
1
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640401
Fernandez
Expression and characterizatio ...
Homo sapiens, Saccharomyces cerevisiae
Glycobiology
12
555-562
2002
-
-
2
-
-
-
-
-
4
-
2
2
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
2
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640400
Rymerson
Dolichol and polyprenol kinase ...
Secale cereale
Biochem. Cell Biol.
70
455-459
1992
1
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640399
Itami
Dolichol kinase in rat sarcopl ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochim. Biophys. Acta
960
374-381
1988
1
-
-
-
-
-
2
2
4
4
-
2
-
163
-
-
-
-
-
6
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
4
4
-
2
-
-
-
-
-
6
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640398
Ravi
-
Characterization of dolichol k ...
Glycine max
Biochim. Biophys. Acta
875
618-625
1986
1
-
-
-
-
-
2
1
2
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
2
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
640395
Sakakihara
Zn2+, not Ca2+, is the most ef ...
Bos taurus, Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
260
15413-15419
1985
-
-
-
-
-
-
2
2
2
14
-
3
-
164
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
2
14
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640396
Sumbilla
Properties of brain dolichol k ...
Bos taurus
Arch. Biochem. Biophys.
238
75-82
1985
4
-
-
-
-
-
4
2
1
2
-
1
-
1
-
-
-
-
-
1
-
2
2
-
-
-
-
-
1
-
-
-
1
-
-
4
-
-
-
-
-
-
-
4
1
2
1
2
-
1
-
-
-
-
-
1
-
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640397
Walter
Dolichol kinase in Ascaris suu ...
Ascaris suum, Onchocerca volvulus
Mol. Biochem. Parasitol.
14
211-217
1985
2
-
-
-
-
-
2
4
2
6
-
-
-
5
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
4
2
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
640394
Gandhi
Calmodulin-dependent enzymic p ...
Tetrahymena pyriformis
Biochem. J.
216
317-323
1983
1
-
-
-
-
-
2
2
-
3
-
1
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
3
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
640392
Allen
Extraction and detergent/lipid ...
Bos taurus
Biochim. Biophys. Acta
712
33-41
1982
3
-
-
-
-
-
5
2
2
4
-
1
-
2
-
-
1
-
-
2
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
5
-
2
2
4
-
1
-
-
-
1
-
2
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
640393
Keller
Subcellular localization and s ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochim. Biophys. Acta
719
118-125
1982
-
-
-
-
-
-
-
-
7
-
-
2
-
164
-
-
1
-
-
3
4
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
1
-
3
4
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640391
Rip
Properties of a dolichol phosp ...
Rattus norvegicus, Rattus norvegicus Wistar
Can. J. Biochem.
58
1051-1057
1980
2
-
-
-
-
-
4
2
1
3
-
2
-
167
-
-
-
-
-
2
-
-
4
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
2
1
3
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
640390
Burton
Enzymatic phosphorylation of d ...
Bos taurus
J. Biol. Chem.
254
7129-7136
1979
2
-
-
-
-
-
1
1
1
3
-
1
-
1
-
-
-
-
-
1
-
1
3
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
1
3
-
1
-
-
-
-
-
1
-
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-