Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.107 extracted from

  • Takahashi, D.; Sakane, F.
    Expression and purification of human diacylglycerol kinase alpha from baculovirus-infected insect cells for structural studies (2018), PeerJ, 6, e5449 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.107

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Rosetta2 (DE3) and baculovirus-infected Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-[(2E)-3-(2-furyl)prop-2-enylidene]-3-[(phenylsulfonyl)amino]2-thioxo-1,3-thiazolidin-4-one i.e. CU-3, ATP competitive inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 ATP isoform DGKalpha, at pH 7.4 and 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.001 mM used in assay conditions. The enzyme is positively regulated by Ca2+ Homo sapiens
Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol Homo sapiens
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P23743
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, Superdex 200 gel filtration or Superdex 75 gel filtration Homo sapiens

Storage Stability

Storage Stability Organism
4°C, purified enzyme in 20 mM Tris-HCl, pH 7.4 with 200 mM NaCl, 3 mM CaCl2, 3 mM MgCl2, 0.5 mM dithiothreitol, and 5% (v/v) glycerol, at least 3 months, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
ATP + 1,2-dioleolyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-dioleolyl-sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, His6-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DGKalpha isoform Homo sapiens
diacylglycerol kinase alpha isoform Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00034
-
 isoform DGKalpha, at pH 7.4 and 30°C Homo sapiens 5-[(2E)-3-(2-furyl)prop-2-enylidene]-3-[(phenylsulfonyl)amino]2-thioxo-1,3-thiazolidin-4-one