Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | isozyme DGK-teta is dependent on an activating accessory protein containing a poly-basic region, the broadening of the pH profile of DGK-theta is also dependent on a PBR-containing activator | Mus musculus | |
phosphatidylserine | broadens the pH-dependent activity when the enzyme is assayed in cytosolic extracts. Phosphatidylserineconcentrates Mg2+ ions at the interface | Mus musculus |
General Stability | Organism |
---|---|
the enzyme exhibits a mean half-life of approximately 5.1 h in cultured cells | Mus musculus |
the enzyme is stabilized during prolonged incubations in the presence of phospholipid vesicles, in a diacylglycerol-independent manner | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by ATP, ADP, sodium pyrophosphate, and beta-glycerophosphate | Mus musculus | |
R59022 | is an effective inhibitor of purified DGK-theta in vitro and in vivo | Mus musculus | |
R59949 | is an effective inhibitor of purified DGK-theta in vitro and in vivo | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, activates. Phosphatidylserine concentrates Mg2+ ions at the interface | Mus musculus | |
Zn2+ | zinc can play a non-catalytic role in DGK regulation. Pre-incubation of DGK-theta with 0.1 mM zinc ions inhibits the enzyme at pH 6.0-7.5 | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
native PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | Mus musculus | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q6P5E8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
central nervous system | isozyme DGK-theta shows a striking predominate localization in the nervous system | Mus musculus | - |
fibroblast | - |
Mus musculus | - |
Neuro-2a cell | - |
Mus musculus | - |
Swiss 3T3 cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | - |
Mus musculus | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DGK-theta | - |
Mus musculus |
diacylglycerol kinase theta | - |
Mus musculus |
type V DGK | - |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the broadening of the pH profile of DGK-theta is also dependent on a PBR-containing activator | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays important roles is the nervous system | Mus musculus |