Literature summary for 2.7.1.107 extracted from

Harada, B.T.; Knight, M.J.; Imai, S.; Qiao, F.; Ramachander, R.; Sawaya, M.R.; Gingery, M.; Sakane, F.; Bowie, J.U.
Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta 1 (2008), Structure, 16, 380-387.

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Application

Application	Comment	Organism
analysis	generation of a soluble mutant selection assay based on a library of random diacylglycerol kinase delta1 mutants of sterile alpha-motif and murine dihydrofolate reductase as the selectable marker	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
C20S	mutation in sterile alpha-motif, mutant forms an oligomer	Mus musculus
D43G	mutation in sterile alpha-motif, mutant is largely monomeric in solution	Mus musculus
E35G	mutation in sterile alpha-motif, mutant is largely monomeric in solution	Mus musculus
G53D	mutation in sterile alpha-motif, mutant is largely monomeric in solution	Mus musculus
K45E	mutation in sterile alpha-motif, mutant forms an oligomer	Mus musculus
K56E	mutation in sterile alpha-motif, mutant is largely monomeric in solution	Mus musculus
additional information	expression of isolated His-tagged sterile alpha-motif domain of diacylglycerol kinase delta1, comprised of residues 1097-1164. The domain forms large aggregates with a molecular weight of 250000 Dalton or greater, while calculated monomer molecular weight is 9500 Dalton	Mus musculus
T57P	mutant with reduced activity, used for construcution of fusion protein for genetic selection of soluble mutants	Mus musculus
V52E	mutation in sterile alpha-motif, mutant is largely monomeric in solution	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	sterile alpha-motif of diacylglycerol kinase delta1 forms helical polymers through a head-to-tail interaction. Disruption of polymerization by mutations constitutively localizes the enzyme to the plasma membrane	Mus musculus	16020	-

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Subunits

Subunits	Comment	Organism
More	sterile alpha-motif of diacylglycerol kinase delta1 forms helical polymers through a head-to-tail interaction. Disruption of polymerization by mutations constitutively localizes the enzyme to the plasma membrane	Mus musculus

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Release 2023.1 (January 2023)