Cloned (Comment) | Organism |
---|---|
expression in COS-7 cell and HeLa cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F369A/F372A | significant decrease in diacylglycerol kinase activity. Mutant cells display reduced uptake of transferrin | Homo sapiens |
F369A/F372A/F748A | significant decrease in diacylglycerol kinase activity. Mutant cells display reduced uptake of transferrin | Homo sapiens |
F748A | diacylglycerol kinase activity similar to that of wild-type. Mutant cells display reduced uptake of transferrin | Homo sapiens |
additional information | diacylglycerol kinase delta has alternative splicing variants, type 1 DGKdelta1 and type 2 DGKdelta2, with calculated molecular masses of 130 and 134 kDa, respectively. HeLa cells express both type 1 and 2 DGKdelta, and COS7 cells express only type 2 DGKdelta. In DGKdelta-knockdown cells uptake of transferrin is reduced. DGKdelta2 is partially co-localized with clathrin or adaptor protein AP2alpha in COS7 cells. Mutants lacking binding ability to AP2alpha as well as kinase-negative mutants cannot compensate for the uptake of transferrin inhibited by siRNA treatment. Overexpression of wild-type diacylglycerol kinase delta2 completely recovers the transferrin uptake | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
diacylglycerol kinase delta2 | - |
Subunits | Comment | Organism |
---|---|---|
More | diacylglycerol kinase delta2 interacts with the platform subdomain of adaptor protein AP2alpha through its DXF-type binding motif. Binding is involved in the transferrin internalization | Homo sapiens |