Literature summary for 2.7.1.107 extracted from

Stathopoulos, V.M.; Coco-Maroney, A.; Wei, C.W.; Goth, M.; Zaricznyj, C.; Macara, I.G.
Identification of two cytosolic diacylglycerol kinase isoforms in rat brain, and in NIH-3T3 and ras-transformed fibroblasts (1990), Biochem. J., 272, 569-575.

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Activating Compound

Activating Compound	Comment	Organism	Structure
phosphatidylcholine	enzyme type II has a preference for phosphatidylcholine as cofactor, enzyme type I can utilize both phosphatidylserine and phosphatidylinositol, but has a lower preference for phosphatidylcholine	Rattus norvegicus
phosphatidylinositol	enzyme type II has a preference for phosphatidylcholine as cofactor, enzyme type I can utilize both phosphatidylserine and phosphatidylinositol, but has a lower preference for phosphatidylcholine	Rattus norvegicus
phosphatidylserine	enzyme type II has a preference for phosphatidylcholine as cofactor, enzyme type I can utilize both phosphatidylserine and phosphatidylinositol, but has a lower preference for phosphatidylcholine	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in NIH 3T3 cells	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.105	-	ATP	pG 7.4, 30°C, enzyme type I	Rattus norvegicus
0.506	-	ATP	pG 7.4, 30°C, enzyme type II	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-
fibroblast	ras-transformed	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1,2-didecanoylglycerol	enzyme type I: activity is 157% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 141% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1,2-didecanoylglycerol 3-phosphate	-	?
ATP + 1,2-didodecanoylglycerol	enzyme type I: activity is 107% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 227% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1,2-didodecanoylglycerol 3-phosphate	-	?
ATP + 1,2-dihexadecanoylglycerol	enzyme type I: activity is 198% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 231% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1,2-dihexadecanoylglycerol 3-phosphate	-	?
ATP + 1,2-dioctanoylglycerol	enzyme type I: activity is 149% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 114% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1,2-dioctanoylglycerol 3-phosphate	-	?
ATP + 1,2-ditetradecanoylglycerol	enzyme type I: activity is 200% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 262% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1,2-ditetradecanoylglycerol 3-phosphate	-	?
ATP + 1-palmitoyl-2-arachidonoyl-sn-glycerol	enzyme type I: activity is 181% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 116% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate	-	?
ATP + 1-palmitoyl-2-linoleoyl-sn-glycerol	enzyme type I: activity is 116% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 86% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + 1-palmitoyl-2-linoleoyl-sn-glycerol 3-phosphate	-	?
ATP + rac-1,2-dioleoylglycerol	-	Rattus norvegicus	ADP + rac-1,2-dioleoylglycerol 3-phosphate	-	?
ATP + sn-1,2-dioleoylglycerol	enzyme type I: activity is 18% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 19% of the activity with rac-1,2-dioleoylglycerol	Rattus norvegicus	ADP + sn-1,2-dioleoylglycerol 3-phosphate	-	?
additional information	enzyme form I and II show a preference for diacylglycerol substrates with saturated acyl chains of 10-12 carbon atoms	Rattus norvegicus	?	-	?

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rattus norvegicus	it remains unclear whether the multiple species, observed by isoelectric focusing represent distinct isoforms of the enzyme, or are modified versions of the type I and type II enzyme	-	additional information

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Release 2023.1 (January 2023)