Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | enzyme type II has a preference for phosphatidylcholine as cofactor, enzyme type I can utilize both phosphatidylserine and phosphatidylinositol, but has a lower preference for phosphatidylcholine | Rattus norvegicus | |
phosphatidylinositol | enzyme type II has a preference for phosphatidylcholine as cofactor, enzyme type I can utilize both phosphatidylserine and phosphatidylinositol, but has a lower preference for phosphatidylcholine | Rattus norvegicus | |
phosphatidylserine | enzyme type II has a preference for phosphatidylcholine as cofactor, enzyme type I can utilize both phosphatidylserine and phosphatidylinositol, but has a lower preference for phosphatidylcholine | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression in NIH 3T3 cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
ATP | pG 7.4, 30°C, enzyme type I | Rattus norvegicus | |
0.506 | - |
ATP | pG 7.4, 30°C, enzyme type II | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
fibroblast | ras-transformed | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-didecanoylglycerol | enzyme type I: activity is 157% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 141% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1,2-didecanoylglycerol 3-phosphate | - |
? | |
ATP + 1,2-didodecanoylglycerol | enzyme type I: activity is 107% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 227% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1,2-didodecanoylglycerol 3-phosphate | - |
? | |
ATP + 1,2-dihexadecanoylglycerol | enzyme type I: activity is 198% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 231% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1,2-dihexadecanoylglycerol 3-phosphate | - |
? | |
ATP + 1,2-dioctanoylglycerol | enzyme type I: activity is 149% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 114% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1,2-dioctanoylglycerol 3-phosphate | - |
? | |
ATP + 1,2-ditetradecanoylglycerol | enzyme type I: activity is 200% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 262% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1,2-ditetradecanoylglycerol 3-phosphate | - |
? | |
ATP + 1-palmitoyl-2-arachidonoyl-sn-glycerol | enzyme type I: activity is 181% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 116% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-palmitoyl-2-linoleoyl-sn-glycerol | enzyme type I: activity is 116% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 86% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + 1-palmitoyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + rac-1,2-dioleoylglycerol | - |
Rattus norvegicus | ADP + rac-1,2-dioleoylglycerol 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | enzyme type I: activity is 18% of the activity with rac-1,2-dioleoylglycerol, enzyme type II: activity is 19% of the activity with rac-1,2-dioleoylglycerol | Rattus norvegicus | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? | |
additional information | enzyme form I and II show a preference for diacylglycerol substrates with saturated acyl chains of 10-12 carbon atoms | Rattus norvegicus | ? | - |
? |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | it remains unclear whether the multiple species, observed by isoelectric focusing represent distinct isoforms of the enzyme, or are modified versions of the type I and type II enzyme | - |
additional information |