Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | amino acids increase beta-D-fructose 2,6-bisphosphate concentration in HeLa and in MCF-7 human cells, molecular mechanisms involved differ from those observed with insulin. Only essential amino acids without glutamine are required to activate the PI3K/mTORC2 and p38/MAPKAPK-2 pathways and to increase the beta-D-fructose 2,6-bisphosphate concentration. The observed effects in signaling and metabolism are produced by a mixture of essential amino acids | Homo sapiens | |
additional information | amino acids increase beta-D-fructose 2,6-bisphosphate concentration in primary cardiomyocytes, molecular mechanisms involved differ from those observed with insulin. Only essential amino acids without glutamine are required to activate the PI3K/mTORC2 and p38/MAPKAPK-2 pathways and to increase the beta-D-fructose 2,6-bisphosphate concentration. The observed effects in signaling and metabolism are produced by a mixture of essential amino acids | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
gene PFKFB2 | Homo sapiens |
gene PFKFB2 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Homo sapiens | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | Rattus norvegicus | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | Rattus norvegicus Sprague-Dawley | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60825 | gene/isozyme PFKFB2 | - |
Rattus norvegicus | Q9JJH5 | gene/isozyme PFKFB2 | - |
Rattus norvegicus Sprague-Dawley | Q9JJH5 | gene/isozyme PFKFB2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | specific phosphorylation of heart 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoenzyme PFKFB2 at Ser483. This activation is mediated by the phosphatidylinositol-4,5-bisphosphate 3-kinase and p38 signaling pathways. Amino acid-activated Akt phosphorylates PFKFB2 at Ser483. Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production | Homo sapiens |
phosphoprotein | specific phosphorylation of heart 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoenzyme PFKFB2 at Ser483. This activation is mediated by the phosphatidylinositol-4,5-bisphosphate 3-kinase and p38 signaling pathways. Amino acid-activated Akt phosphorylates PFKFB2 at Ser483. Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiomyocyte | primary | Rattus norvegicus | - |
heart | heart isozyme PFKFB2 | Homo sapiens | - |
heart | heart isozyme PFKFB2 | Rattus norvegicus | - |
HeLa cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Homo sapiens | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus Sprague-Dawley | ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase | - |
Homo sapiens |
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase | - |
Rattus norvegicus |
PFKFB2 | - |
Homo sapiens |
PFKFB2 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Homo sapiens |
7.5 | 8 | assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production | Homo sapiens |
malfunction | Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production | Rattus norvegicus |