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Literature summary for 2.7.1.105 extracted from

  • Yalcin, A.; Clem, B.F.; Simmons, A.; Lane, A.; Nelson, K.; Clem, A.L.; Brock, E.; Siow, D.; Wattenberg, B.; Telang, S.; Chesney, J.
    Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases (2009), J. Biol. Chem., 284, 24223-24232.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
both parent and double mutant (K472A/K473A) constructs are transformed into the BL21 strain of Escherichia coli, overexpression of PFKFB3 causes no change in glucose metabolism but rather a marked increase in cell proliferation Homo sapiens

Protein Variants

Protein Variants Comment Organism
K472A/K473A cytoplasmic mutant Homo sapiens
R75A/R76A kinase inactive Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-
nucleus PFKFB3 splice variant 5, the carboxyl terminus is required for this localization Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Homo sapiens
-
ADP + beta-D-fructose 2,6-bisphosphate regulation of glycolysis ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16875
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HCT-116 cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
MDA-MB-231 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
nuclei isolated from cells transfected with wild type PFKFB3 exhibit 6-phosphofructo-2-kinase activity that is significantly higher than the nuclei obtained from both vector and K472A/K473A-transfected cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate regulation of glycolysis ?

Synonyms

Synonyms Comment Organism
6-phosphofructo-2-kinase
-
Homo sapiens
PFKFB3
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens ectopic expression of wild type PFKFB3 increases phosphorylation of the cell cycle inhibitor p27, a member of the Kip/Cip family of proteins and a universal inhibitor of cyclin-dependent kinases and the cell cycle, at threonine 187 and decreases total p27 protein levels additional information

General Information

General Information Comment Organism
malfunction PFKFB3 enzymes are activated in human cancers Homo sapiens
physiological function PFKFB3 has a role in nuclear signaling Homo sapiens