Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.105 extracted from

  • Baez, M.; Rodriguez, P.H.; Babul, J.; Guixe, V.
    Structural and functional roles of Cys-238 and Cys-295 in Escherichia coli phosphofructokinase-2 (2003), Biochem. J., 376, 277-283.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.105

Inhibitors

Inhibitors Comment Organism Structure
pyrene maleimide incorporation of 2 mol per mol of enzyme subunit, modifiying Cys-238 and Cys-295, leads to rapid inactivation, MgATP2- protects Cys295, modification of Cys238 does not abolish activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of native and pyrene maleimide modified enzyme Escherichia coli
0.042
-
 MgATP2- native enzyme Escherichia coli
0.044
-
 MgATP2- C238-pyrene maleimide modified enzyme Escherichia coli
0.058
-
 beta-D-fructose 6-phosphate native enzyme Escherichia coli
0.063
-
 beta-D-fructose 6-phosphate C238-pyrene maleimide modified enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ as MgATP2- Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Escherichia coli
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate ordered bi bi mechanism, structural and functional roles of Cys-238 and Cys-295 Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Escherichia coli ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
MgATP2- + beta-D-fructose 6-phosphate
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer wild-type enzyme in presence of MgATP2-, and C238-pyrene maleimide modified enzyme in presence of beta-D-fructose 6-phosphate and ATP4- Escherichia coli
monomer C238-pyrene maleimide modified enzyme in absence of MgATP2- Escherichia coli
tetramer wild-type enzyme in absence of ligands, and C238-pyrene maleimide modified enzyme in presence of MgATP2- Escherichia coli

Synonyms

Synonyms Comment Organism
Pfk-2
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9240
-
 MgATP2- native enzyme Escherichia coli
9240
-
 beta-D-fructose 6-phosphate native enzyme Escherichia coli
9320
-
 MgATP2- C238-pyrene maleimide modified enzyme Escherichia coli
9320
-
 beta-D-fructose 6-phosphate C238-pyrene maleimide modified enzyme Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP as MgATP2- Escherichia coli