Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Sparus aurata | |
citrate | phosphorylation enhances sensitivity | Sparus aurata | |
additional information | no inhibition by lactate, glyceraldehyde 3-phosphate, beta-D-fructose 1,6-bisphosphate | Sparus aurata | |
phosphoenolpyruvate | mixed-type inhibitory effect, phosphorylation enhances sensitivity | Sparus aurata | |
sn-glycerol 3-phosphate | most potent inhibitor of phosphorylated liver enzyme, phosphorylation enhances sensitivity | Sparus aurata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data of native and phosphorylated enzyme | Sparus aurata | |
0.018 | - |
ATP | pH 7.5, 30°C, in presence of phosphate | Sparus aurata | |
0.156 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C, with ATP, in presence of phosphate | Sparus aurata | |
0.226 | - |
ATP | pH 7.5, 30°C, absence of phosphate | Sparus aurata | |
1.4 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C, with ATP, absence of phosphate | Sparus aurata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Sparus aurata |
98000 | - |
gel filtration | Sparus aurata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Sparus aurata | - |
ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sparus aurata | - |
teleost fish | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Sparus aurata |
Purification (Comment) | Organism |
---|---|
liver | Sparus aurata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Sparus aurata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.068 | - |
- |
Sparus aurata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Sparus aurata | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Sparus aurata | ADP + beta-D-fructose 2,6-bisphosphate | via phosphorylenzyme intermediate | r | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Sparus aurata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Sparus aurata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sparus aurata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Sparus aurata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
phosphoenolpyruvate | noncompetitive to ATP, pH 7.5, 30°C | Sparus aurata | |
0.53 | - |
phosphoenolpyruvate | noncompetitive to fructose 6-phosphate, pH 7.5, 30°C | Sparus aurata | |
0.55 | - |
citrate | pH 7.5, 30°C | Sparus aurata |