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Literature summary for 2.7.1.105 extracted from

  • Rider, M.H.; Foret, D.; Hue, L.
    Comparison of purified bovine heart and rat liver 6-phosphofructo-2-kinase. Evidence for distinct isoenzymes (1985), Biochem. J., 231, 193-196.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.105

Inhibitors

Inhibitors Comment Organism Structure
citrate heart enzyme is more sensitive than liver enzyme Bos taurus
citrate heart enzyme is more sensitive than liver enzyme Rattus norvegicus
additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme Bos taurus
additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme Rattus norvegicus
sn-glycerol 3-phosphate heart enzyme is less sensitive than liver Bos taurus
sn-glycerol 3-phosphate heart enzyme is less sensitive than liver Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 beta-D-fructose 6-phosphate
-
 Rattus norvegicus
0.05
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C Bos taurus
0.1
-
 ATP pH 7.5, 30°C, MgATP Rattus norvegicus
0.1
-
 ATP pH 7.5, 30°C, MgATP Bos taurus
0.14
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgATP2 Rattus norvegicus
Mg2+ MgATP2 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 2 * 52000, SDS-PAGE Bos taurus
58000
-
 2 * 58000, SDS-PAGE Rattus norvegicus
97000
-
 gel filtration Bos taurus
112500
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-D-fructose 6-phosphate Rattus norvegicus responsible for regulation of fructose 2,6-bisphosphate-concentration ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
ATP + beta-D-fructose 6-phosphate Bos taurus responsible for regulation of fructose 2,6-bisphosphate-concentration ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Rattus norvegicus
phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Bos taurus
phosphoprotein rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme Rattus norvegicus
phosphoprotein rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme Bos taurus

Purification (Commentary)

Purification (Comment) Organism
heart Bos taurus
liver Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Rattus norvegicus
-
heart
-
 Bos taurus
-
liver
-
 Rattus norvegicus
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
ATP + beta-D-fructose 6-phosphate responsible for regulation of fructose 2,6-bisphosphate-concentration Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
ATP + beta-D-fructose 6-phosphate responsible for regulation of fructose 2,6-bisphosphate-concentration Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Rattus norvegicus
dimer 2 * 52000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Bos taurus
7.1
-
-
 Rattus norvegicus