Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is induced by growing on D-hamamelose | Kluyvera cryocrescens |
General Stability | Organism |
---|---|
no stabilization by dithioerythritol, mercaptoethanol, glycerol or bovine serum albumin | Kluyvera cryocrescens |
purified enzyme is very labile, 4 mM D-hamamelose, added to storage buffer, stabilizes | Kluyvera cryocrescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | above 7 mM: significant substrate inhibition | Kluyvera cryocrescens | |
hamamelose | above 5 mM: significant substrate inhibition | Kluyvera cryocrescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
ATP | at 30°C, pH 7.5 | Kluyvera cryocrescens | |
3 | - |
D-hamamelose | at 30°C, pH 7.5 | Kluyvera cryocrescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
gel filtration | Kluyvera cryocrescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-hamamelose | Kluyvera cryocrescens | initial step of the catabolic reaction sequence for dissimilation of hamamelose | ADP + hamamelose 2'-phosphate | - |
? | |
ATP + D-hamamelose | Kluyvera cryocrescens 627 | initial step of the catabolic reaction sequence for dissimilation of hamamelose | ADP + hamamelose 2'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyvera cryocrescens | - |
grown on D-hamamelose | - |
Kluyvera cryocrescens 627 | - |
grown on D-hamamelose | - |
Purification (Comment) | Organism |
---|---|
partial, 51fold | Kluyvera cryocrescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | 6.6 | at 30°C, pH 7.5 | Kluyvera cryocrescens |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, within 10 days, complete loss of activity | Kluyvera cryocrescens |
low temperatures, 4 mM hamamelose, protein concentration not lower than 0.2 mg/ml, stable | Kluyvera cryocrescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-hamamelitol | poor substrate | Kluyvera cryocrescens | ADP + D-hamamelitol 2'-phosphate | - |
? | |
ATP + D-hamamelitol | poor substrate | Kluyvera cryocrescens 627 | ADP + D-hamamelitol 2'-phosphate | - |
? | |
ATP + D-hamamelose | specific for hamamelose | Kluyvera cryocrescens | ADP + hamamelose 2'-phosphate | - |
? | |
ATP + D-hamamelose | initial step of the catabolic reaction sequence for dissimilation of hamamelose | Kluyvera cryocrescens | ADP + hamamelose 2'-phosphate | - |
? | |
ATP + D-hamamelose | specific for hamamelose | Kluyvera cryocrescens 627 | ADP + hamamelose 2'-phosphate | - |
? | |
ATP + D-hamamelose | initial step of the catabolic reaction sequence for dissimilation of hamamelose | Kluyvera cryocrescens 627 | ADP + hamamelose 2'-phosphate | - |
? | |
additional information | no substrates: D-glucose, D-glucosamine, D-fructose, D-mannose, D-galactose, D-arabinose, D-xylose | Kluyvera cryocrescens | ? | - |
? | |
additional information | no substrates: D-glucose, D-glucosamine, D-fructose, D-mannose, D-galactose, D-arabinose, D-xylose | Kluyvera cryocrescens 627 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kluyvera cryocrescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Kluyvera cryocrescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.5 | 10% of maximal activity at pH 6.6, 80% of maximal activity at pH 8.5 | Kluyvera cryocrescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
optimal enzyme stability in the crude extract at | Kluyvera cryocrescens |