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Literature summary for 2.7.1.100 extracted from

  • Nakano, T.; Saito, Y.; Yokota, A.; Ashida, H.
    Plausible novel ribose metabolism catalyzed by enzymes of the methionine salvage pathway in Bacillus subtilis (2013), Biosci. Biotechnol. Biochem., 77, 1104-1107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mtnK, encoded in the mtnKA operon Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the Km for ribose is 80times higher than for S-methyl-5-thio-D-ribose Bacillus subtilis
0.06
-
S-methyl-5-thio-D-ribose pH 8.0, 25°C Bacillus subtilis
4.8
-
D-ribose pH 8.0, 25°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + S-methyl-5-thio-D-ribose Bacillus subtilis physiological substrate ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene mtnK
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose
-
Bacillus subtilis ADP + D-ribose 1-phosphate
-
?
ATP + S-methyl-5-thio-D-ribose best substrate Bacillus subtilis ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?
ATP + S-methyl-5-thio-D-ribose physiological substrate Bacillus subtilis ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?
additional information the enzyme has significant ribose kinase activity, it has a hydrophobic pocket that specifically recognizes the methylthio group of the substrate. MTK shows a 9fold higher Vmax for ribose kinase activity than for S-methyl-5-thio-D-ribose kinase activity, but also has a several fold higher Km for ribose Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
MtnK
-
Bacillus subtilis
MTR kinase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 35 ribose kinase activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 ribose kinase activity Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis

General Information

General Information Comment Organism
evolution although most bacteria, yeasts, and animals have the complete methionine salvage pathway, MSP enzymes, these organisms have 5-methylthioadenosine phosphorylase, MtnP, instead of MtnK Bacillus subtilis
metabolism the enzyme catalyze the first reaction of a ribose metabolic pathway, overview Bacillus subtilis