Cloned (Comment) | Organism |
---|---|
gene mtnK, encoded in the mtnKA operon | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km for ribose is 80times higher than for S-methyl-5-thio-D-ribose | Bacillus subtilis | |
0.06 | - |
S-methyl-5-thio-D-ribose | pH 8.0, 25°C | Bacillus subtilis | |
4.8 | - |
D-ribose | pH 8.0, 25°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + S-methyl-5-thio-D-ribose | Bacillus subtilis | physiological substrate | ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene mtnK | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose | - |
Bacillus subtilis | ADP + D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | best substrate | Bacillus subtilis | ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | physiological substrate | Bacillus subtilis | ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
additional information | the enzyme has significant ribose kinase activity, it has a hydrophobic pocket that specifically recognizes the methylthio group of the substrate. MTK shows a 9fold higher Vmax for ribose kinase activity than for S-methyl-5-thio-D-ribose kinase activity, but also has a several fold higher Km for ribose | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtnK | - |
Bacillus subtilis |
MTR kinase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | ribose kinase activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | ribose kinase activity | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | although most bacteria, yeasts, and animals have the complete methionine salvage pathway, MSP enzymes, these organisms have 5-methylthioadenosine phosphorylase, MtnP, instead of MtnK | Bacillus subtilis |
metabolism | the enzyme catalyze the first reaction of a ribose metabolic pathway, overview | Bacillus subtilis |