Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | strong upregulation of enzyme expression during sulfur starvation | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Arabidopsis thaliana |
DNA and amino acid sequence determination and analysis, overvexpression of a slightly modified enzyme in Escherichia coli strain BL21 as GST-fusion protein | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | insertional mutagenesis results in inability of the plants to grow on methylthioadenosine as asupplemntal sulfur source, knockout plants show unimpaired growth under standard conditions | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | cooperative kinetics | Oryza sativa | |
0.0168 | - |
S-methyl-5-thio-D-ribose | pH 8.5, 37°C | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Oryza sativa | |
Mg2+ | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48600 | - |
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE | Oryza sativa |
50000 | - |
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + S-methyl-5-thio-D-ribose | Oryza sativa | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | Arabidopsis thaliana | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
additional information | Oryza sativa | the enzyme regulation is related to the sulfur metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C6D2 | - |
- |
Oryza sativa | Q7XR61 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off | Oryza sativa |
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
root | strong upregulation of enzyme expression during sulfur starvation | Oryza sativa | - |
shoot | strong upregulation of enzyme expression during sulfur starvation | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + S-methyl-5-thio-D-ribose | - |
Oryza sativa | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | - |
Arabidopsis thaliana | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | Oryza sativa | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
ATP + S-methyl-5-thio-D-ribose | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | Arabidopsis thaliana | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
additional information | the enzyme regulation is related to the sulfur metabolism | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
methylthioribose kinase | - |
Oryza sativa |
methylthioribose kinase | - |
Arabidopsis thaliana |
MTK | - |
Arabidopsis thaliana |
MTK1 | - |
Oryza sativa |
MTR kinase | - |
Arabidopsis thaliana |
MTR kinase1 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa |
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Oryza sativa |
8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryza sativa | |
ATP | - |
Arabidopsis thaliana |