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Literature summary for 2.7.1.100 extracted from

  • Ferro, A.J.; Barrett, A.; Shapiro, S.K.
    5-Methylthioribose kinase. A new enzyme involved in the formation of methionine from 5-methylthioribose (1978), J. Biol. Chem., 253, 6021-6025.
    View publication on PubMed
Search for more literature for 2.7.1.100

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required for activity, maximal activity at 20 mM Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
ADP 52% inhibition at equimolar concentrations with ATP Klebsiella aerogenes
CTP in the presence of ATP Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0081
-
 5-methylthioribose pH 7.3, 37°C Klebsiella aerogenes
0.074
-
 ATP pH 7.3, 37°C Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 5 mM Klebsiella aerogenes
Mg2+ divalent metal ion is strictely required for activity, Mg2+ is most effective Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-methylthioribose Klebsiella aerogenes may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine ADP + 5-methylthioribose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-200, DEAE-cellulose Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.028
-
-
 Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol, at least 5 mg/ml, stable Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-methylthioribose no activity with GTP and UTP Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
 ?
ATP + 5-methylthioribose may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
 ?
CTP + 5-methylthioribose 20% of activity with ATP Klebsiella aerogenes CDP + 5-methylthioribose 1-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Klebsiella aerogenes