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Literature summary for 2.7.1.1 extracted from

  • Troncoso-Ponce, M.A.; Rivoal, J.; Dorion, S.; Moisan, M.C.; Garces, R.; Martinez-Force, E.
    Cloning, biochemical characterization and expression of a sunflower (Helianthus annuus L.) hexokinase associated with seed storage compounds accumulation (2011), J. Plant Physiol., 168, 299-308.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
a truncated version, without the 24 N-terminal residues, is heterologously expressed in Escherichia coli Helianthus annuus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Helianthus annuus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01911
-
 D-mannose pH 7.0, 22°C Helianthus annuus
0.02164
-
 D-mannose pH 7.6, 22°C Helianthus annuus
0.02339
-
 D-mannose pH 8.2, 22°C Helianthus annuus
0.03054
-
 ATP pH 7.6, 22°C Helianthus annuus
0.0338
-
 D-glucose pH 7.0, 22°C Helianthus annuus
0.034
-
 D-glucose pH 8.2, 22°C Helianthus annuus
0.03872
-
 D-glucose pH 7.6, 22°C Helianthus annuus
0.06948
-
 ATP pH 8.2, 22°C Helianthus annuus
0.08816
-
 ATP pH 7.0, 22°C Helianthus annuus
0.654
-
 GTP pH 8.2, 22°C Helianthus annuus
0.717
-
 GTP pH 7.0, 22°C Helianthus annuus
0.788
-
 GTP pH 7.6, 22°C Helianthus annuus
1.162
-
 UTP pH 7.0, 22°C Helianthus annuus
1.598
-
 UTP pH 7.6, 22°C Helianthus annuus
1.791
-
 UTP pH 8.2, 22°C Helianthus annuus
1.993
-
 CTP pH 7.6, 22°C Helianthus annuus
2.317
-
 CTP pH 8.2, 22°C Helianthus annuus
3.625
-
 D-fructose pH 7.0, 22°C Helianthus annuus
4.567
-
 D-fructose pH 7.6, 22°C Helianthus annuus
5.145
-
 D-fructose pH 8.2, 22°C Helianthus annuus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52600
-
 predicted from cDNA Helianthus annuus

Organism

Organism UniProt Comment Textmining
Helianthus annuus B8K2W7
-
-

Purification (Commentary)

Purification (Comment) Organism
using immobilized metal ion affinity chromatography Helianthus annuus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Helianthus annuus
-
root
-
 Helianthus annuus
-
seed
-
 Helianthus annuus
-
stem
-
 Helianthus annuus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.3
-
 pH 7.6, 22°C Helianthus annuus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
 Helianthus annuus ADP + D-fructose 6-phosphate
-
 ?
ATP + D-glucose
-
 Helianthus annuus ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose
-
 Helianthus annuus ADP + D-mannose 6-phosphate
-
 ?
CTP + D-glucose
-
 Helianthus annuus CDP + D-glucose 6-phosphate
-
 ?
GTP + D-glucose
-
 Helianthus annuus GDP + D-glucose 6-phosphate
-
 ?
UTP + D-glucose
-
 Helianthus annuus UDP + D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Helianthus annuus

Synonyms

Synonyms Comment Organism
HaHXK1
-
 Helianthus annuus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
-
 Helianthus annuus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 enzyme retains 80% activity at 40°C Helianthus annuus
48
-
 enzyme shows no activity above 48°C Helianthus annuus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 optimal pH Helianthus annuus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 enzyme shows only 40% activity at pH 7.0 Helianthus annuus

Expression

Organism Comment Expression
Helianthus annuus higher levels in heterotrophic tissues, developing seeds and roots, than in photosynthetic ones. A time course of HXK activity and expression in seeds shows that the highest HXK levels are found at the early stages of reserve compounds, lipids and proteins accumulation up