Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-tagged fusion protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L146R | mutant shows a 100fold reduced kcat and a 40fold increase in [S]0.5 value for D-glucose. Km (ATP) is slightly increased compared to wild-type | Homo sapiens |
T228M | mutant shows a 9000fold reduced activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
ATP | wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 0.5 mM D-glucose | Homo sapiens | |
0.16 | - |
ATP | wild-type, 37°C, pH not specified in the puclication | Homo sapiens | |
0.16 | - |
ATP | wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 60 mM D-glucose | Homo sapiens | |
0.24 | - |
ATP | mutant L146R, 37°C, pH not specified in the puclication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-glucose = ADP + D-glucose 6-phosphate | using intrinsic tryptophan fluorescence a conformational change induced by the binding of adenine nucleotides to human pancreatic glucokinase is determined. The molecular dynamic simulations indicate that the binding triggers molecular motion of the flexible surface /active site loop and partial closure of the interdomain active site cleft. The modelled structure of the hGK-ATP binary complex shows the residue contacts involved in ATP binding at the active site | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucokinase | - |
Homo sapiens |
hGK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
D-glucose | mutant T228M, 37°C, pH not specified in the puclication | Homo sapiens | |
0.61 | - |
ATP | mutant L146R, 37°C, pH not specified in the puclication | Homo sapiens | |
0.7 | - |
ATP | wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 0.5 mM D-glucose | Homo sapiens | |
0.77 | - |
D-glucose | mutant L146R, 37°C, pH not specified in the puclication | Homo sapiens | |
67.6 | - |
D-glucose | wild-type, 37°C, pH not specified in the puclication | Homo sapiens | |
68.4 | - |
ATP | wild-type, 37°C, pH not specified in the puclication | Homo sapiens | |
68.4 | - |
ATP | wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 60 mM D-glucose | Homo sapiens |