Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-fusion protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A379T | kcat(1/sec): 61.2, D-glucose S05 (mM): 12.3, Km (ATP): 0.87 mM, relative inhibition of glucokinase activity through GKRP alone: 17% and GKRP plus 10 microM sorbitol 6-phosphate: 53% | Homo sapiens |
A456V | kcat(1/sec): 65.6, D-glucose S05 (mM): 1.91, Km (ATP): 0.35 mM, relative inhibition of glucokinase activity through GKRP alone: 11% and GKRP plus 10 microM sorbitol 6-phosphate: 28% | Homo sapiens |
C213R | kcat(1/sec): 50, D-glucose S05 (mM): 21.6, Km (ATP): 0.89 mM, relative inhibition of glucokinase activity through GKRP alone: 19% and GKRP plus 10 microM sorbitol 6-phosphate: 43% | Homo sapiens |
C252Y | kcat(1/sec): 29.3, D-glucose S05 (mM): 31.6, Km (ATP): 0.75 mM, relative inhibition of glucokinase activity through GKRP alone: 4% and GKRP plus 10 microM sorbitol 6-phosphate: 4% | Homo sapiens |
E442K | kcat(1/sec): 52.6, D-glucose S05 (mM): 5.24, Km (ATP): 1.5 mM, relative inhibition of glucokinase activity through GKRP alone: 14% and GKRP plus 10 microM sorbitol 6-phosphate: 40% | Homo sapiens |
G68K | kcat(1/sec): 43.2, D-glucose S05 (mM): 2.34, Km (ATP): 0.39 mM, relative inhibition of glucokinase activity through GKRP alone: 2% and GKRP plus 10 microM sorbitol 6-phosphate: 20.5% | Homo sapiens |
G68V | kcat(1/sec): 62.7, D-glucose S05 (mM): 2.2, Km (ATP): 0.31 mM, relative inhibition of glucokinase activity through GKRP alone: 15% and GKRP plus 10 microM sorbitol 6-phosphate: 32.5% | Homo sapiens |
G72R | kcat(1/sec): 29.8, D-glucose S05 (mM): 7.38, Km (ATP): 0.72 mM, relative inhibition of glucokinase activity through GKRP alone: 5% and GKRP plus 10 microM sorbitol 6-phosphate: 6.5% | Homo sapiens |
K140E | kcat(1/sec): 40, D-glucose S05 (mM): 10.8, Km (ATP): 0.35 mM, relative inhibition of glucokinase activity through GKRP alone: 9% and GKRP plus 10 microM sorbitol 6-phosphate: 10.5% | Homo sapiens |
K414E | kcat(1/sec): 19.9, D-glucose S05 (mM): 5.69, Km (ATP): 1.53 mM, relative inhibition of glucokinase activity through GKRP alone: 13.5% and GKRP plus 10 microM sorbitol 6-phosphate: 49% | Homo sapiens |
M197E | kcat(1/sec): 17.7, D-glucose S05 (mM): 41.6, Km (ATP): 0.4 mM, relative inhibition of glucokinase activity through GKRP alone: 13.5% and GKRP plus 10 microM sorbitol 6-phosphate: 18.5% | Homo sapiens |
M197I | kcat(1/sec): 58.1, D-glucose S05 (mM): 2.49, Km (ATP): 1.39 mM, relative inhibition of glucokinase activity through GKRP alone: 47% and GKRP plus 10 microM sorbitol 6-phosphate: 59% | Homo sapiens |
M197I/A397T | kcat(1/sec): 50.2, D-glucose S05 (mM): 5.81, Km (ATP): 2.71 mM, relative inhibition of glucokinase activity through GKRP alone: 18% and GKRP plus 10 microM sorbitol 6-phosphate: 37% | Homo sapiens |
M197L | kcat(1/sec): 62.6, D-glucose S05 (mM): 4.03, Km (ATP): 1.31 mM, relative inhibition of glucokinase activity through GKRP alone: 15% and GKRP plus 10 microM sorbitol 6-phosphate: 37% | Homo sapiens |
M298K | kcat(1/sec): 37.7, D-glucose S05 (mM): 10.8, Km (ATP): 3.32 mM, relative inhibition of glucokinase activity through GKRP alone: 20% and GKRP plus 10 microM sorbitol 6-phosphate: 44% | Homo sapiens |
P417R | kcat(1/sec): 48.3, D-glucose S05 (mM): 6.59, Km (ATP): 2.08 mM, relative inhibition of glucokinase activity through GKRP alone: 10% and GKRP plus 10 microM sorbitol 6-phosphate: 39% | Homo sapiens |
S263P | kcat(1/sec): 44.6, D-glucose S05 (mM): 9.7, Km (ATP): 0.63 mM, relative inhibition of glucokinase activity through GKRP alone: 19% and GKRP plus 10 microM sorbitol 6-phosphate: 49.5% | Homo sapiens |
S336L | kcat(1/sec): 2.46, D-glucose S05 (mM): 4.75, Km (ATP): 12.6 mM | Homo sapiens |
S64P | kcat(1/sec): 83.3, D-glucose S05 (mM): 2.07, Km (ATP): 0.32 mM, relative inhibition of glucokinase activity through GKRP alone: 5% and GKRP plus 10 microM sorbitol 6-phosphate: 4% | Homo sapiens |
S64Y | kcat(1/sec): 114, D-glucose S05 (mM): 1.89, Km (ATP): 1.59 mM, relative inhibition of glucokinase activity through GKRP alone: 19% and GKRP plus 10 microM sorbitol 6-phosphate: 15% | Homo sapiens |
T65I | kcat(1/sec): 22.6, D-glucose S05 (mM): 1.69, Km (ATP): 0.57 mM, relative inhibition of glucokinase activity through GKRP alone: 7.5% and GKRP plus 10 microM sorbitol 6-phosphate: 23% | Homo sapiens |
V389L | kcat(1/sec): 67.1, D-glucose S05 (mM): 3.45, Km (ATP): 0.76 mM, relative inhibition of glucokinase activity through GKRP alone: 67% and GKRP plus 10 microM sorbitol 6-phosphate: 75% | Homo sapiens |
V452L | kcat(1/sec): 122, D-glucose S05 (mM): 2.27, Km (ATP): 0.53 mM, relative inhibition of glucokinase activity through GKRP alone: 19% and GKRP plus 10 microM sorbitol 6-phosphate: 38% | Homo sapiens |
V455M | kcat(1/sec): 61.8, D-glucose S05 (mM): 3.24, Km (ATP): 0.38 mM, relative inhibition of glucokinase activity through GKRP alone: 26% and GKRP plus 10 microM sorbitol 6-phosphate: 52% | Homo sapiens |
V62M | kcat(1/sec): 47.2, D-glucose S05 (mM): 5.9, Km (ATP): 0.52 mM, relative inhibition of glucokinase activity through GKRP alone: 8.5% and GKRP plus 10 microM sorbitol 6-phosphate: 11.5% | Homo sapiens |
V91L | kcat(1/sec): 60.6, D-glucose S05 (mM): 1.66, Km (ATP): 0.48 mM, relative inhibition of glucokinase activity through GKRP alone: 6% and GKRP plus 10 microM sorbitol 6-phosphate: 22.5% | Homo sapiens |
Y214A | kcat(1/sec): 117, D-glucose S05 (mM): 1.41, Km (ATP): 0.92 mM, relative inhibition of glucokinase activity through GKRP alone: 24% and GKRP plus 10 microM sorbitol 6-phosphate: 53% | Homo sapiens |
Y214A/V452A | kcat(1/sec): 23.1, D-glucose S05 (mM): 0.55, Km (ATP): 1.42 mM, relative inhibition of glucokinase activity through GKRP alone: 11.5% and GKRP plus 10 microM sorbitol 6-phosphate: 20% | Homo sapiens |
Y214C | kcat(1/sec): 65.3, D-glucose S05 (mM): 1.35, Km (ATP): 1.08 mM, relative inhibition of glucokinase activity through GKRP alone: 19% and GKRP plus 10 microM sorbitol 6-phosphate: 41% | Homo sapiens |
Y215A | kcat(1/sec): 44.7, D-glucose S05 (mM): 2.07, Km (ATP): 0.58 mM, relative inhibition of glucokinase activity through GKRP alone: 6% and GKRP plus 10 microM sorbitol 6-phosphate: 4.5% | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucokinase-regulatory protein | GK regulatory protein, relative inhibition of glucokinase activity through GKRP alone wild-type: 32.5% and GKRP plus 10 microM sorbitol 6-phosphate: 55 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant 454-Ala | Homo sapiens | |
0.31 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68V | Homo sapiens | |
0.32 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64P | Homo sapiens | |
0.35 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A456V | Homo sapiens | |
0.35 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K140E | Homo sapiens | |
0.38 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V455M | Homo sapiens | |
0.39 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68K | Homo sapiens | |
0.4 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197E | Homo sapiens | |
0.46 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, wild-type | Homo sapiens | |
0.48 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V91L | Homo sapiens | |
0.52 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M | Homo sapiens | |
0.53 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V452L | Homo sapiens | |
0.57 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant T65I | Homo sapiens | |
0.58 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y215A | Homo sapiens | |
0.63 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S263P | Homo sapiens | |
0.72 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G72R | Homo sapiens | |
0.75 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C252Y | Homo sapiens | |
0.76 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V389L | Homo sapiens | |
0.87 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A379T | Homo sapiens | |
0.89 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C213R | Homo sapiens | |
0.92 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A | Homo sapiens | |
1.08 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214C | Homo sapiens | |
1.31 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197L | Homo sapiens | |
1.39 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I | Homo sapiens | |
1.42 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A/V452A | Homo sapiens | |
1.5 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant E442K | Homo sapiens | |
1.53 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K414E | Homo sapiens | |
1.59 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64Y | Homo sapiens | |
2.08 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant P417R | Homo sapiens | |
2.71 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I/A379T | Homo sapiens | |
3.32 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M298K | Homo sapiens | |
12.6 | - |
ATP | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S336L | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35557 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucokinase 1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.46 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S336L | Homo sapiens | |
17.7 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197E | Homo sapiens | |
19.9 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K414E | Homo sapiens | |
22.6 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant T65I | Homo sapiens | |
23.1 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A/V452A | Homo sapiens | |
29.3 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C252Y | Homo sapiens | |
29.8 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G72R | Homo sapiens | |
37.7 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M298K | Homo sapiens | |
40 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K140E | Homo sapiens | |
43.2 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68K | Homo sapiens | |
44.6 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S263P | Homo sapiens | |
44.7 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y215A | Homo sapiens | |
47.2 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M | Homo sapiens | |
48.3 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant P417R | Homo sapiens | |
50 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C213R | Homo sapiens | |
50.2 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I/A379T | Homo sapiens | |
52.2 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant 454-Ala | Homo sapiens | |
52.6 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant E442K | Homo sapiens | |
58.1 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I | Homo sapiens | |
60.6 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V91L | Homo sapiens | |
61.2 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A379T | Homo sapiens | |
61.8 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V455M | Homo sapiens | |
62.6 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197L | Homo sapiens | |
62.7 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68V | Homo sapiens | |
62.8 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, wild-type | Homo sapiens | |
65.3 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214C | Homo sapiens | |
65.6 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A456V | Homo sapiens | |
67.1 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V389L | Homo sapiens | |
83.3 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64P | Homo sapiens | |
114 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64Y | Homo sapiens | |
117 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A | Homo sapiens | |
122 | - |
D-glucose | pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V452L | Homo sapiens |