Literature summary for 2.7.1.1 extracted from

Petit, P.; Antoine, M.; Ferry, G.; Boutin, J.A.; Lagarde, A.; Gluais, L.; Vincentelli, R.; Vuillard, L.
The active conformation of human glucokinase is not altered by allosteric activators (2011), Acta Crystallogr. Sect. D, 67, 929-935.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
first structures of a glucokinase-glucose complex without activator, of glucokinase-glucose-AMP-PNP and of glucokinase-glucose-AMP-PNP with a bound activator are reported. All structures are extremely similar, thus demonstrating that binding of GK activators does not result in conformational changes of the active protein but in stabilization of the active form of glucokinase	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35557	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography followed by ion-exchange chromatography on a HiTrap Q FF column and gel filtration on a Superdex 200 column	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-glucose	-	Homo sapiens	ADP + D-glucose 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
glucokinase 1	-	Homo sapiens

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Release 2023.1 (January 2023)