Activating Compound | Comment | Organism | Structure |
---|---|---|---|
LY-2121260 | addition of 0.02 mM LY-2121260 results in the loss of positive cooperativity with glucose and in activation of glucokinase catalysis increasing both kcat and the apparent glucose affinity | Homo sapiens | |
N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine | addition of 0.02 mM N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine results in the loss of positive cooperativity with glucose and in activation of glucokinase catalysis increasing both kcat and the apparent glucose affinity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
D-glucose | wild type enzyme, at 25°C, in the presence of 0.02 mM activator LY-2121260 | Homo sapiens | |
0.7 | - |
ATP | wild type enzyme, at 25°C | Homo sapiens | |
0.76 | - |
D-glucose | wild type enzyme, at 25°C, in the presence of 0.02 mM activator N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Hitrap Q FF column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreatic beta cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hexokinase Dor | - |
Homo sapiens |
hexokinase IV | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54 | - |
ATP | wild type enzyme, at 25°C | Homo sapiens | |
64 | - |
D-glucose | wild type enzyme, at 25°C, in the presence of 0.02 mM activator N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine | Homo sapiens | |
75 | - |
D-glucose | wild type enzyme, at 25°C, in the presence of 0.02 mM activator LY-2121260 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |