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Literature summary for 2.7.1.1 extracted from

  • Antoine, M.; Boutin, J.A.; Ferry, G.
    Binding kinetics of glucose and allosteric activators to human glucokinase reveal multiple conformational states (2009), Biochemistry, 48, 5466-5482.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
LY-2121260 addition of 0.02 mM LY-2121260 results in the loss of positive cooperativity with glucose and in activation of glucokinase catalysis increasing both kcat and the apparent glucose affinity Homo sapiens
N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine addition of 0.02 mM N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine results in the loss of positive cooperativity with glucose and in activation of glucokinase catalysis increasing both kcat and the apparent glucose affinity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
D-glucose wild type enzyme, at 25°C, in the presence of 0.02 mM activator LY-2121260 Homo sapiens
0.7
-
ATP wild type enzyme, at 25°C Homo sapiens
0.76
-
D-glucose wild type enzyme, at 25°C, in the presence of 0.02 mM activator N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Hitrap Q FF column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreatic beta cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
Homo sapiens ADP + D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
hexokinase Dor
-
Homo sapiens
hexokinase IV
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54
-
ATP wild type enzyme, at 25°C Homo sapiens
64
-
D-glucose wild type enzyme, at 25°C, in the presence of 0.02 mM activator N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine Homo sapiens
75
-
D-glucose wild type enzyme, at 25°C, in the presence of 0.02 mM activator LY-2121260 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens