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Literature summary for 2.7.1.1 extracted from

  • Estalella, I.; Garcia-Gimeno, M.A.; Marina, A.; Castano, L.; Sanz, P.
    Biochemical characterization of novel glucokinase mutations isolated from Spanish maturity-onset diabetes of the young (MODY2) patients (2008), J. Hum. Genet., 53, 460-466.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
glutathione S-transferase (GST)-GlkB fusion proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
A379V mutations is associated with mature-onset diabetes of the young, type 2 (MODY2). Vmax is 65% of maximal activity, Km-value for ATP is 1.5fold higher than wild-type enzyme Homo sapiens
C233R mutations is associated with mature-onset diabetes of the young, type 2 (MODY2). Mutation affects a critical residue of the active center of the enzyme and rendered a protein with undetectable enzymatic activity Homo sapiens
E265K mutations is associated with mature-onset diabetes of the young, type 2 (MODY2). Vmax is 86% of maximal activity, Km-value for ATP is 1.13fold lower than wild-type enzyme Homo sapiens
K420E mutations is associated with mature-onset diabetes of the young, type 2 (MODY2). Vmax is 94% of maximal activity, Km-value for ATP is 1.1fold lower than wild-type enzyme Homo sapiens
V182L mutations is associated with mature-onset diabetes of the young, type 2 (MODY2). Vmax is 38% of maximal activity, Km-value for ATP is 2.8fold lower than wild-type enzyme Homo sapiens
Y61S mutations is associated with mature-onset diabetes of the young, type 2 (MODY2). Vmax is 16% of maximal activity, Km-value for ATP is 2.1fold lower than wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 ATP mutant enzyme V182L, glutathione S-transferase glucokinase B fusion protein Homo sapiens
0.21
-
 ATP mutant enzyme Y61S, glutathione S-transferase glucokinase B fusion protein Homo sapiens
0.4
-
 ATP mutant enzyme E265K, glutathione S-transferase glucokinase B fusion protein Homo sapiens
0.41
-
 ATP mutant enzyme K420E, glutathione S-transferase glucokinase B fusion protein Homo sapiens
0.45
-
 ATP wild-type glutathione S-transferase glucokinase B fusion protein Homo sapiens
0.69
-
 ATP mutant enzyme A379V, glutathione S-transferase glucokinase B fusion protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q53Y25
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione S-transferase (GST)-GlkB fusion proteins, wild-type and mutant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GlkB
-
 Homo sapiens
glucokinase B
-
 Homo sapiens
hexokinase IV
-
 Homo sapiens